A Genetic Screen for the Identification of Thiamin Metabolic Genes

A genetic screen was developed for the identification of genes related to thiamin biosynthesis and degradation. Genes conferring resistance to bacimethrin or 4-amino-2-trifluoromethyl-5-hydroxymethylpyrimidine were selected from Escherichia coli and Bacillus subtilis genomic libraries. Hits from the...

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Bibliographic Details
Published in:The Journal of biological chemistry 2004-10, Vol.279 (42), p.43555-43559
Main Authors: Lawhorn, Brian G, Gerdes, Svetlana Y, Begley, Tadhg P
Format: Article
Language:English
Subjects:
Bacillus subtilis
Bacillus subtilis - drug effects
Bacillus subtilis - genetics
Cloning, Molecular
Escherichia coli
Escherichia coli - drug effects
Escherichia coli - genetics
Genomic Library
Phosphorylation
Pyrimidines - pharmacology
Thiamine - biosynthesis
Thiamine - genetics
Thiamine - metabolism
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Summary:A genetic screen was developed for the identification of genes related to thiamin biosynthesis and degradation. Genes conferring resistance to bacimethrin or 4-amino-2-trifluoromethyl-5-hydroxymethylpyrimidine were selected from Escherichia coli and Bacillus subtilis genomic libraries. Hits from the selection included the known thiamin biosynthetic genes thiC , thiE , and dxs as well as five genes of previously unknown function ( E. coli yjjX, yajO, ymfB, and cof and B. subtilis yveN ). The gene products YmfB and Cof catalyze the hydrolysis of 4-amino-2-methyl-5-hydroxymethylpyrimidine pyrophosphate to 4-amino-2-methyl-5-hydroxymethylpyrimidine phosphate. YmfB also converts thiamin pyrophosphate into thiamin phosphate.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M404284200