Renin-angiotensin gene polymorphisms in relation to severe chronic periodontitis

• Published in: Journal of clinical periodontology 2009-03, Vol.36 (3), p.204-211
• Main Authors: Gürkan, Ali, Emingil, Gülnur, Saygan, Buket Han, Atilla, Gül, Köse, Timur, Baylas, Haluk, Berdeli, Afig
• Format: Article
• Language: English
• Subjects: ACE; Adult; AGT; Analysis of Variance; Angiotensinogen - genetics; AT1R; Biological and medical sciences; Case-Control Studies; Chi-Square Distribution; Chronic Periodontitis - genetics; Dentistry; Facial bones, jaws, teeth, parodontium: diseases, semeiology; Female; Genetic Predisposition to Disease; Humans; Male; Medical sciences; Middle Aged; Non tumoral diseases; Otorhinolaryngology. Stomatology; Peptidyl-Dipeptidase A - genetics; periodontitis; polymorphism; Receptor, Angiotensin, Type 1 - genetics; Reference Values; Renin-Angiotensin System - genetics; Severity of Illness Index; Turkey
• ISSN: 0303-6979; 1600-051X
• DOI: 10.1111/j.1600-051X.2008.01379.x

Aim: Evidence suggests that the ultimate product of the renin–angiotensin system (RAS), angiotensin II, exerts inflammatory actions. The present study aimed to evaluate the inter‐relation between gene polymorphisms of the RAS components; angiotensin converting enzyme (ACE), angiotensinogen (AGT) and angiotensin II type‐I receptor (AT1R), and severe chronic periodontitis (CP). Material and Methods: DNA was obtained from peripheral blood of 90 CP patients and 126 periodontally healthy subjects, and the clinical parameters were recorded. ACE I/D, AGT M235T and AT1R A1166C polymorphisms were genotyped by the PCR–RFLP method. Chi‐square, anova and logistic regression methods were used in statistical analyses. Results: The frequency of the ACE D allele was significantly lower in the CP group than the healthy group (pcorr=0.015). CP subjects exhibited increased C allele carriage and C allele frequency of the AT1R gene (pcorr=0.03 and pcorr=0.03, respectively). All clinical parameters of CP patients were found to be similar in variant allele‐carrying and non‐carrying subjects (p>0.05). Conclusions: The present findings suggest that ACE I/D and AT1R polymorphisms might be associated with susceptibility to CP but not with disease severity. The D allele of ACE I/D might be associated with decreased, whereas the C variant of AT1R A1166C might be associated with an elevated risk for CP in Turkish population.