Upregulation of Macrophage Migration Inhibitory Factor Gene Expression in Stroke

MIF has been implicated to function in many inflammatory processes. This study examined whether MIF expression was affected in stroke and its underlying molecular mechanism. ELISA and qRT-PCR were used to detect MIF protein and mRNA in PBMCs from stroke patients, the ischemic rat brains, and control...

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Bibliographic Details
Published in:Stroke (1970) 2009-03, Vol.40 (3), p.973-976
Main Authors: LIJUAN WANG, ZIS, Odysseus, WEIHONG SONG, GUIXIAN MA, ZHIXIN SHAN, XIONG ZHANG, SHUO WANG, CHENGBO DAI, JIEHAO ZHAO, QIUXIONG LIN, SHUGUANG LIN
Format: Article
Language:English
Subjects:
Aged
Animals
Base Sequence
Biological and medical sciences
Blotting, Western
Brain Ischemia - genetics
Brain Ischemia - metabolism
Enzyme-Linked Immunosorbent Assay
Female
Headache. Facial pains. Syncopes. Epilepsia. Intracranial hypertension. Brain oedema. Cerebral palsy
Humans
Macrophage Migration-Inhibitory Factors - biosynthesis
Macrophage Migration-Inhibitory Factors - genetics
Male
Medical sciences
Molecular Sequence Data
Nervous system (semeiology, syndromes)
Neurology
Promoter Regions, Genetic - genetics
Rats
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Sequence Tagged Sites
Stroke - genetics
Up-Regulation
Vascular diseases and vascular malformations of the nervous system
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Summary:MIF has been implicated to function in many inflammatory processes. This study examined whether MIF expression was affected in stroke and its underlying molecular mechanism. ELISA and qRT-PCR were used to detect MIF protein and mRNA in PBMCs from stroke patients, the ischemic rat brains, and controls. A MIF promoter assay under hypoxia was performed. MIF protein and mRNA were significantly increased in stroke patients. Increasing levels of MIF were correlated to the severity of stroke and peaked 24 hours after stroke. MIF was significantly upregulated in focal ischemic rat brains. The activity of the human MIF promoter was significantly increased under hypoxia compared to normoxia. MIF gene expression is upregulated after stroke, and hypoxia signaling plays an important role in upregulation of MIF expression under stroke.
ISSN:0039-2499
1524-4628
DOI:10.1161/STROKEAHA.108.530535