Allergy-Preventive Effects of Hibiscus mutabilis ‘Versicolor’ and a Novel Allergy-Preventive Flavonoid Glycoside

• Published in: Biological & Pharmaceutical Bulletin 2009/03/01, Vol.32(3), pp.509-512
• Main Authors: Iwaoka, Emiko, Oku, Hisae, Takahashi, Yumi, Ishiguro, Kyoko
• Format: Article
• Language: English
• Subjects: allergy-preventive effect; Animals; Anti-Allergic Agents - isolation & purification; Anti-Allergic Agents - pharmacology; blood flow; Flavonoids - isolation & purification; Flavonoids - pharmacology; flavonol triglycoside; Glycosides - isolation & purification; Glycosides - pharmacology; hen-egg white lysozyme; Hibiscus - chemistry; Hibiscus mutabilis; Hibiscus mutabilis ‘versicolor’; Male; Mice; Muramidase - immunology; mutabiloside; Plant Extracts - chemistry; Plant Extracts - pharmacology; Platelet Aggregation - drug effects; Regional Blood Flow - drug effects; Tail - blood supply
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.32.509

Allergy-preventive activity was demonstrated for the MeOH extract (HM) of the petals of Hibiscus mutabilis L. ‘versicolor’ MAKINO in a continuing search for allergy-preventive substances from natural sources, using the in vivo assay method. This assay system uses monitoring of a decrease in the blood flow at the tail vein of mice subjected to hen egg-white lysozyme (HEL) sensitization. By bioassay-directed fractionation, a new flavonol triglycoside, quercetin 3-O-[β-D-xylopyranosyl(1→2)-α-L-rhamnopyranosyl(1→6)]-β-D-galactopyranoside (1: mutabiloside), was isolated, together with four known flavonols identified as quercetin 3-O-[β-D-xylopyranosyl(1→2)]-β-D-galactopyranoside (2) and kaempferol 3-O-[β-D-xylopyranosyl(1→2)]-β-D-galactopyranoside (3), quercetin (4) and hyperoside (5). The structure of the new flavonol 1 was elucidated by spectroscopic methods. Among these flavonol derivatives, compounds 1 and 2 showed significant allergy-preventive effects.