Analysis of impurities in streptomycin and dihydrostreptomycin by hydrophilic interaction chromatography/electrospray ionization quadrupole ion trap/time-of-flight mass spectrometry

Impurities in streptomycin (STR) and dihydrostreptomycin (DHS) were investigated by hydrophilic interaction chromatography/electrospray ionization quadrupole ion trap/time‐of‐flight mass spectrometry (HILIC/ESI‐QIT/TOFMS). Samples were separated on a fused‐core silica column (100 mm × 2.1 mm i.d., p...

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Bibliographic Details
Published in:Rapid communications in mass spectrometry 2009-03, Vol.23 (6), p.907-914
Main Author: Kawano, Shin-ichi
Format: Article
Language:English
Subjects:
Chromatography, High Pressure Liquid - methods
Dihydrostreptomycin Sulfate - analysis
Dihydrostreptomycin Sulfate - chemistry
Drug Contamination - prevention & control
Hydrophobic and Hydrophilic Interactions
Spectrometry, Mass, Electrospray Ionization - methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Streptomycin - analysis
Streptomycin - chemistry
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Summary:Impurities in streptomycin (STR) and dihydrostreptomycin (DHS) were investigated by hydrophilic interaction chromatography/electrospray ionization quadrupole ion trap/time‐of‐flight mass spectrometry (HILIC/ESI‐QIT/TOFMS). Samples were separated on a fused‐core silica column (100 mm × 2.1 mm i.d., particle size: 2.7 µm) with isocratic elution using 200 mM ammonium formate buffer (pH 4.5) and acetonitrile as mobile phase. Constant neutral loss survey in accurate mass measurement was carried out by QIT/TOFMS. Formulae, chemical structures of impurities in an STR sample were suggested with supporting results on the probable pathways of STR biosynthesis by Streptomyces griseus. Copyright © 2009 John Wiley & Sons, Ltd.
ISSN:0951-4198
1097-0231
DOI:10.1002/rcm.3936