Ultrastructural localization of parkin in the rat brainstem, thalamus and basal ganglia

The parkin gene encodes a 52 kd putative E3 ubiquitin-protein ligase involved in an autosomal recessive form of early onset parkinsonism. Parkin ultrastructural localization was studied by immunohistochemistry in the adult rat brain and in a parkin inducible PC12 cell line (HS22). In the rat brain,...

Full description

Saved in:
Bibliographic Details
Published in:Journal of Neural Transmission 2004-10, Vol.111 (10-11), p.1209-1218
Main Authors: Mouatt-Prigent, A, Muriel, M-P, Gu, W-J, El Hachimi, K H, Lücking, C B, Brice, A, Hirsch, E C
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - pharmacology
Basal Ganglia - metabolism
Basal Ganglia - ultrastructure
Brain Stem - metabolism
Brain Stem - ultrastructure
Dendrites - drug effects
Dendrites - metabolism
Dendrites - ultrastructure
Doxycycline - pharmacology
Endoplasmic Reticulum - drug effects
Endoplasmic Reticulum - metabolism
Endoplasmic Reticulum - ultrastructure
Immunohistochemistry
Male
Mitochondria - drug effects
Mitochondria - metabolism
Mitochondria - ultrastructure
Neuroglia - drug effects
Neuroglia - metabolism
Neuroglia - ultrastructure
Neurons - drug effects
Neurons - metabolism
Neurons - ultrastructure
PC12 Cells
Rats
Rats, Wistar
Synapses - drug effects
Synapses - metabolism
Synapses - ultrastructure
Thalamus - metabolism
Thalamus - ultrastructure
Ubiquitin-Protein Ligases - biosynthesis
Ubiquitin-Protein Ligases - genetics
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The parkin gene encodes a 52 kd putative E3 ubiquitin-protein ligase involved in an autosomal recessive form of early onset parkinsonism. Parkin ultrastructural localization was studied by immunohistochemistry in the adult rat brain and in a parkin inducible PC12 cell line (HS22). In the rat brain, parkin immunoreactivity was detected in neuronal and glial cell bodies and in nerve processes. In the neurons, it was mostly localized on the periphery of large vesicles, some rare mitochondria and endoplasmic reticulum in the cell bodies, and on the periphery of large vesicles in the dendrites and terminals of the neurons. In addition, parkin immunoreactivity was also found around synaptic vesicles in the presynaptic elements of some axons. In HS22 cells over-expressing parkin, the distribution of the protein was similar to that observed in the perikarya of the labeled neurons.
ISSN:0300-9564
1435-1463
DOI:10.1007/s00702-004-0144-9