Immunocytochemical Assessment of p53 Protein to Detect Malignancy in Increased Cell-Yield Brush Cytology from the Biliopancreatic Tree

Background Malignancies arising from the biliopancreatic tree are often diagnostic challenges for the gastroenterologist and the pathologist, especially when strictures without masses are present. Aim To evaluate the diagnostic yield of p53 immunocytology for the detection of malignancies in materia...

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Bibliographic Details
Published in:Digestive diseases and sciences 2009-04, Vol.54 (4), p.789-792
Main Authors: Villanacci, Vincenzo, Cestari, Renzo, Giulini, Stefano, Cengia, Paolo, Missale, Guido, Berenzi, Angiola, Rossi, Elisa, Bonardi, Massimo, Baiocchi, Luca, Bassotti, Gabrio
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Biliary Tract Neoplasms - diagnosis
Biliary Tract Neoplasms - metabolism
Biochemistry
Biological and medical sciences
Biomarkers, Tumor - metabolism
Carcinoma - diagnosis
Carcinoma - metabolism
Feeding. Feeding behavior
Female
Fundamental and applied biological sciences. Psychology
Gastroenterology
Hepatology
Humans
Immunohistochemistry - methods
Male
Medicine
Medicine & Public Health
Middle Aged
Oncology
Original Article
Pancreatic Neoplasms - diagnosis
Pancreatic Neoplasms - metabolism
Retrospective Studies
Sensitivity and Specificity
Transplant Surgery
Tumor Suppressor Protein p53 - metabolism
Vertebrates: anatomy and physiology, studies on body, several organs or systems
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Summary:Background Malignancies arising from the biliopancreatic tree are often diagnostic challenges for the gastroenterologist and the pathologist, especially when strictures without masses are present. Aim To evaluate the diagnostic yield of p53 immunocytology for the detection of malignancies in material obtained by biliopancreatic tree brushing by means of an increased cell-yield procedure. Patients and Methods Cytologic specimens obtained from biliary and pancreatic tree brushing in 24 patients with biliary strictures suspected for malignancy were assessed by conventional Papanicolau staining and p53 immunocytochemistry. Results Papanicolau staining detected 67% and p53 87% of the malignancies in the study group. p53 immunocytology displayed excellent sensitivity, specificity, and diagnostic accuracy. Conclusions p53 immunocytology may represent a useful diagnostic tool in the detection of malignancies from biliary and pancreatic tree brushing, especially when using an increasing cell-yield procedure.
ISSN:0163-2116
1573-2568
DOI:10.1007/s10620-008-0431-7