Protective Effects of Caffeic Acid Phenethyl Ester on Intestinal Ischemia-Reperfusion Injury

• Published in: Digestive diseases and sciences 2009-04, Vol.54 (4), p.738-744
• Main Authors: Yildiz, Yuksel, Serter, Mukadder, Ek, Rauf Onur, Ergin, Kemal, Cecen, Serpil, Demir, Ece Mine, Yenisey, Cigdem
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants - metabolism; Biochemistry; Biological and medical sciences; Caffeic Acids - therapeutic use; Cardiology. Vascular system; Catalase - metabolism; Feeding. Feeding behavior; Fundamental and applied biological sciences. Psychology; Gastroenterology; Gastroenterology. Liver. Pancreas. Abdomen; Glutathione - metabolism; Glutathione Peroxidase - metabolism; Hepatology; Intestinal Diseases - drug therapy; Intestinal Diseases - enzymology; Intestinal Diseases - pathology; Intestinal Mucosa - pathology; Jejunum - pathology; Lipid Peroxidation; Male; Malondialdehyde - metabolism; Medical sciences; Medicine; Medicine & Public Health; Oncology; Original Article; Other diseases. Semiology; Peroxidase - metabolism; Phenylethyl Alcohol - analogs & derivatives; Rats; Rats, Wistar; Reperfusion Injury - drug therapy; Reperfusion Injury - enzymology; Reperfusion Injury - pathology; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Superoxide Dismutase - metabolism; Transplant Surgery; Tumor Necrosis Factor-alpha - blood; Vertebrates: anatomy and physiology, studies on body, several organs or systems
• ISSN: 0163-2116; 1573-2568
• DOI: 10.1007/s10620-008-0405-9

Aim Intestinal ischemia reperfusion (IR) causes tissue injury in two ways, starting a pro-inflammatory cascade and oxidative stress. The aim of this study was to investigate the possible protective effects of caffeic acid phenethyl ester (CAPE), which has antioxidant and anti-inflammatory properties, against intestinal IR injury in rats. Materials and Methods Forty male Wistar-Albino rats were divided into five groups: Sham, IR, IR plus ethanol (vehicle), IR plus 10 mg/kg (IR + 10CAPE), and 30 mg/kg CAPE (IR + 30CAPE) at the 30-min ischemic period. Intestines were exteriorized and the superior mesenteric artery was occluded for 45-min ischemia and then the clamp was removed for 120-min reperfusion. After the experiment, the intestines were removed for biochemical and light microscopic examinations. Additionally, blood samples were taken for plasma TNF-α measurement. Results The TBARS levels of the IR and IR + Ethanol groups were higher than the Sham group (P < 0.05). Both CAPE treatments decreased TBARS levels in comparison with the IR group (P < 0.05). In both CAPE-treated groups, while the superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were increased compared to all other groups, which was similarly the case for the CAT activity compared to the Sham and IR + Ethanol groups (P < 0.05). There were no significant differences between GSH levels of all study groups. The TNF-α levels of the IR and IR + Ethanol groups were non-significantly increased compared to the Sham group (P > 0.05). The TNF-α levels of 10 and 30 mg/kg CAPE groups were non-significantly decreased compared to the IR group (P > 0.05). The tissue MPO activities of the IR and IR + Ethanol groups were higher than the Sham group (P < 0.05). The MPO activities of the IR + 10CAPE and IR + 30CAPE groups were not significantly different from the Sham group (P > 0.05). There was necrosis of mucosa in the IR and IR + Ethanol groups in light microscopic evaluations. Those changes were significantly reversed by 30 mg/kg CAPE treatment. Conclusion The intestinal IR injury may be reversed by anti-inflammatory and antioxidant actions of the CAPE. However, 30 mg/kg CAPE treatment may be more efficient in preventing intestinal IR injury in rats.