Gene frequency distribution of the BoLA-DRB3 locus in Saavedreno Creole dairy cattle

The objective of this study is to describe the gene frequency distribution of the bovine lymphocyte antigen (BoLA)-DRB3 locus in Saavedreño Creole dairy cattle and to compare it with previously reported patterns in other cattle breeds. One hundred and twenty-five Saavedreño Creole dairy cattle were...

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Bibliographic Details
Published in:Biochemical genetics 2004-08, Vol.42 (7-8), p.231-240
Main Authors: Ripoli, M.V, Liron, J.P, Luca, J.C. de, Rojas, F, Dulout, F.N, Giovambattista, G
Format: Article
Language:English
Subjects:
Alleles
Animals
Bos taurus
Cattle
cattle breeds
Clonal selection
Dairies
Dairy cattle
DNA - metabolism
Frequency distribution
Gene Frequency
Genetics
Genome
Genotype
Heterozygosity
Heterozygote
histocompatibility antigens
Histocompatibility Antigens Class II - genetics
loci
lymphocyte antigens
Lymphocytes
Polymerase Chain Reaction
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
Population studies
Restriction fragment length polymorphism
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Summary:The objective of this study is to describe the gene frequency distribution of the bovine lymphocyte antigen (BoLA)-DRB3 locus in Saavedreño Creole dairy cattle and to compare it with previously reported patterns in other cattle breeds. One hundred and twenty-five Saavedreño Creole dairy cattle were genotyped for the BoLA-DRB3.2 allele by polymerase chain reaction and restriction fragment length polymorphism. Twenty-two out of 53 previously identified BoLA-DRB3.2 alleles were detected, with gene frequencies ranging from 0.4 to 16.8%. Seventy percent of the variation corresponded to the seven most frequent alleles (BoLA-DRB3.2*7, *8, *11, *16, *27, *36, and *37). The studied population exhibits a high degree of expected heterozygosity (he = 0.919). The FIS index did not show significant deviation from Hardy-Weinberg equilibrium. However, the neutrality test showed an even gene frequency distribution. This result could be better explained assuming balancing selection instead of neutral or positive selection for one or a few alleles. In conclusion, the results of this study demonstrated that BoLA-DRB3.2 is a highly polymorphic locus in Saavedreño Creole dairy cattle, with significant variation in allele frequency among cattle breeds.
ISSN:0006-2928
1573-4927
DOI:10.1023/b:bigi.0000034427.66887.fe