Adaptational modification of serine and threonine metabolism in the liver to essential amino acid deficiency in rats

It is known that plasma serine and threonine concentrations are elevated in rats chronically fed an essential amino acid deficient diet, but the underlying mechanisms including related gene expressions or serine and threonine concentrations in liver remained to be elucidated. We fed rats lysine or v...

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Bibliographic Details
Published in:Amino acids 2009-03, Vol.36 (3), p.555-562
Main Authors: Nagao, Kenji, Bannai, Makoto, Seki, Shinobu, Mori, Masato, Takahashi, Michio
Format: Article
Language:English
Subjects:
Amino acids
Amino Acids, Essential - administration & dosage
Amino Acids, Essential - deficiency
Analytical Chemistry
Animals
Biochemical Engineering
Biochemistry
Biomedical and Life Sciences
Diet
Diets
Elevated
Elevation
Food, Formulated
Gene Expression
L-Serine Dehydratase - biosynthesis
Life Sciences
Liver
Liver - enzymology
Liver - metabolism
Lysine
Metabolism
Neurobiology
Original Article
Phosphoglycerate Dehydrogenase - biosynthesis
Proteomics
Rats
Rats, Wistar
RNA, Messenger - metabolism
Rodents
Serine - blood
Serine - metabolism
Threonine - blood
Threonine - metabolism
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Summary:It is known that plasma serine and threonine concentrations are elevated in rats chronically fed an essential amino acid deficient diet, but the underlying mechanisms including related gene expressions or serine and threonine concentrations in liver remained to be elucidated. We fed rats lysine or valine deficient diet for 4 weeks and examined the mRNA expressions of serine synthesising (3-phosphoglycerate dehydrogenase, PHGDH) and serine/threonine degrading enzymes (serine dehydratase, SDS) in the liver. Dietary deficiency induced marked elevation of hepatic serine and threonine levels associated with enhancement of PHGDH mRNA expression and repression of SDS mRNA expression. Increases in plasma serine and threonine levels due to essential amino acid deficiency in diet were caused by marked increases in hepatic serine and threonine levels. Proteolytic responses to the amino acid deficiency may be lessened by storing amino radicals as serine and inducing anorexia through elevation of threonine.
ISSN:0939-4451
1438-2199
DOI:10.1007/s00726-008-0117-7