Marchantin C, a novel microtubule inhibitor from liverwort with anti-tumor activity both in vivo and in vitro

Abstract Microtubules are long-standing targets in cancer chemotherapy. Previously, we reported that marchantin C triggers apoptosis of human tumor cells. We show here that marchantin C induced cell cycle arrest at G2 /M phase in A172 and HeLa cells. In addition, marchantin C decreased the quantity...

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Bibliographic Details
Published in:Cancer letters 2009-04, Vol.276 (2), p.160-170
Main Authors: Shi, Yan-qiu, Zhu, Chang-jun, Yuan, Hui-qing, Li, Bo-qin, Gao, Jie, Qu, Xian-jun, Sun, Bin, Cheng, Yan-na, Li, Song, Li, Xia, Lou, Hong-Xiang
Format: Article
Language:English
Subjects:
Animals
Antineoplastic Agents, Phytogenic - pharmacology
Apoptosis
Bibenzyls - pharmacology
Catechols - pharmacology
Cell culture
Cell cycle
Cell cycle arrest
Cell division
Cell Division - drug effects
Cell Line, Tumor
Cytotoxicity
Ethers, Cyclic - pharmacology
Female
Flow cytometry
Free radicals
G2 Phase - drug effects
Hematology, Oncology and Palliative Medicine
Humans
Macrocyclic bisbibenzyl
Marchantin C
Mice
Mice, Inbred BALB C
Microscopy
Microtubule inhibitor
Microtubules - drug effects
Microtubules - metabolism
Phenyl Ethers - pharmacology
Polymerization
Rodents
Software
Tumor cell apoptosis
Uterine Cervical Neoplasms - drug therapy
Xenograft Model Antitumor Assays
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Summary:Abstract Microtubules are long-standing targets in cancer chemotherapy. Previously, we reported that marchantin C triggers apoptosis of human tumor cells. We show here that marchantin C induced cell cycle arrest at G2 /M phase in A172 and HeLa cells. In addition, marchantin C decreased the quantity of microtubules in a time- and dose-dependent manner in these cells. Exposure of purified bovine brain tubulin to marchantin C inhibited polymerization of gross tubulin in vitro. Moreover, marchantin C potently suppressed the growth of human cervical carcinoma xenografts in nude mice. Marchantin C-treated xenografts showed decreased microtubules, Bcl-2 and increased cyclin B1, Bax, caspase-3, indicating that marchantin C possess the same ability to induce microtubules depolymerization and tumor cell apoptosis in tumor-bearing mice as in vitro . In conclusion, marchantin C is a novel microtubule inhibitor that induces mitotic arrest of tumor cells and suppresses tumor cell growth, exhibiting promising antitumor therapeutic potential.
ISSN:0304-3835
1872-7980
DOI:10.1016/j.canlet.2008.11.004