Birth of mice from vitrified/warmed 2-cell embryos transported at a cold temperature

Cryopreservation of 2-cell embryos is an effective technology for storage of genetically engineered mouse strains. Transport of genetically engineered mice between laboratories has frequently been performed using such cryopreserved 2-cell embryos. However, the receiving laboratory requires proficien...

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Bibliographic Details
Published in:Cryobiology 2009-04, Vol.58 (2), p.196-202
Main Authors: Takeo, Toru, Kaneko, Takehito, Haruguchi, Yukie, Fukumoto, Kiyoko, Machida, Hiromi, Koga, Mika, Nakagawa, Yoshiko, Takeshita, Yumi, Matsuguma, Toyokazu, Tsuchiyama, Shuuji, Shimizu, Norihiko, Hasegawa, Takanori, Goto, Motohito, Miyachi, Hitoshi, Anzai, Masayuki, Nakatsukasa, Ena, Nomaru, Koji, Nakagata, Naomi
Format: Article
Language:English
Subjects:
Animals
Cold storage
Cryopreservation - instrumentation
Cryopreservation - methods
Cryoprotective Agents - chemistry
Cryoprotective Agents - pharmacology
Embryo, Mammalian - cytology
Embryo, Mammalian - embryology
Female
Hydrogen-Ion Concentration
M2 medium
Male
Mice
Mice, Inbred C57BL
Pregnancy
Pregnancy, Animal
Time Factors
Transport
Transportation
Vitrified/warmed 2-cell embryo
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Summary:Cryopreservation of 2-cell embryos is an effective technology for storage of genetically engineered mouse strains. Transport of genetically engineered mice between laboratories has frequently been performed using such cryopreserved 2-cell embryos. However, the receiving laboratory requires proficient skills and special instruments to obtain live young from cryopreserved and transported embryos. Therefore, in this study, we tried to address the storage and transport of vitrified/warmed 2-cell embryos at a cold temperature. In cold storage experiments, the development rates of 2-cell embryos stored in M2 medium for 24, 48 and 72 h into blastocysts were relatively high (83%, 63% and 43%, respectively). Although, 2-cell embryos stored in PB1 and mWM maintained the developmental potency for 24 h, the rates were markedly decreased to low levels after 48 h (PB1: 0%; mWM: 5%). In transport experiments, many pups were obtained from vitrified/warmed 2-cell embryos transported at a cold temperature in all receiving laboratories (incidence of successful development: 49%; 249/511). In summary, short-term storage and transport of vitrified/warmed 2-cell embryos in M2 medium at a cold temperature can maintain their ability to develop into live young.
ISSN:0011-2240
1090-2392
DOI:10.1016/j.cryobiol.2008.12.011