Purkinje cell functions in the in vitro cerebellum isolated from neonatal rats in a block with the pons and medulla

The cerebellum dissected from neonatal rats in a block including the pons and medulla and maintained in a modified Krebs solution provides a unique preparation of brain tissue with preserved major fiber connections. Electrophysiological and optical recordings revealed that, at postnatal days 4–8 (P4...

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Bibliographic Details
Published in:Neuroscience research 2004-11, Vol.50 (3), p.361-367
Main Authors: Arata, Akiko, Ito, Masao
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Cerebellum
In vitro preparation
In Vitro Techniques
Inferior olive
LTD
Medulla Oblongata - cytology
Medulla Oblongata - growth & development
Neonatal rat
Optical imaging
Parallel fiber
Pons - cytology
Pons - growth & development
Purkinje Cells - cytology
Purkinje Cells - physiology
Rats
Rats, Wistar
Synaptic Transmission - physiology
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Summary:The cerebellum dissected from neonatal rats in a block including the pons and medulla and maintained in a modified Krebs solution provides a unique preparation of brain tissue with preserved major fiber connections. Electrophysiological and optical recordings revealed that, at postnatal days 4–8 (P4–P8), Purkinje cells in the lateral portion of the cerebellum responded to the stimulation of climbing fiber afferents at the inferior olive and of parallel fibers at the posterior vermis, and that the combination of these inputs induced long-term depression. These observations and pharmacological testings indicate that certain functional features of Purkinje cells mature around P5 in the lateral portion of the cerebellum.
ISSN:0168-0102
1872-8111
DOI:10.1016/j.neures.2004.08.011