Sensitive DNA biosensor based on a long-period grating formed on the side-polished fiber surface

We demonstrate a sensitive DNA biosensor based on a long period grating (LPG) formed by a photolithograph process on the surface of a side-polished fiber. The biomolecules of the biosensor were immobilized on the silica surface between LPG patterns. The resonance wavelength was red-shifted after the...

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Bibliographic Details
Published in:Optics express 2009-03, Vol.17 (5), p.3855-3860
Main Authors: Jang, Hyun Soo, Park, Kwang No, Kim, Jun P, Sim, Sang Jun, Kwon, Oh J, Han, Young-Geun, Lee, Kyung Shik
Format: Article
Language:English
Subjects:
Base Sequence
Biosensing Techniques - instrumentation
Biosensing Techniques - statistics & numerical data
DNA - analysis
DNA - genetics
DNA Probes - genetics
DNA, Single-Stranded - genetics
Equipment Design
Optical Fibers
Optical Phenomena
Photography
Polylysine
Sensitivity and Specificity
Silicon Dioxide
Surface Properties
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Summary:We demonstrate a sensitive DNA biosensor based on a long period grating (LPG) formed by a photolithograph process on the surface of a side-polished fiber. The biomolecules of the biosensor were immobilized on the silica surface between LPG patterns. The resonance wavelength was red-shifted after the binding of the poly-L-lysine, probe ssDNA and target ssDNA to the sensor surface. The overall wavelength shift after the successful DNA hybridization was 1.82 nm. The proposed LPG-based DNA biosensor is approximately 2.5 times more sensitive than the previously reported fiber grating-based DNA biosensors.
ISSN:1094-4087
DOI:10.1364/OE.17.003855