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Microsatellite instability in gastric MALT lymphoma
The role of microsatellite instability and defects in DNA mismatch repair mechanism in the pathogenesis of gastric lymphoma of mucosa-associated lymphoid tissue (MALT) type is still controversial, as both negative and positive findings have been reported. This may be explained mainly by arbitrary se...
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Published in: | Modern pathology 2004-11, Vol.17 (11), p.1407-1413 |
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description | The role of microsatellite instability and defects in DNA mismatch repair mechanism in the pathogenesis of gastric lymphoma of mucosa-associated lymphoid tissue (MALT) type is still controversial, as both negative and positive findings have been reported. This may be explained mainly by arbitrary selection of the tested loci, the use of various techniques of microsatellite instability analysis and by different definitions of replication error positive phenotype. The aim of our study was to evaluate the instability at selected microsatellite markers using the GeneScan Analysis Software. DNA from paraffin-embedded tissue blocks of 13 previously untreated patients with localized gastric MALT lymphoma was extracted. Five microsatellite markers, which are located in hMSH2, hMLH1, P16, APC and MLL loci, were selected from the genetic database. We found genetic instability in tumors of 9/13 patients with gastric MALT lymphoma (69%). Seven of them had replication-error-positive phenotype (54%). Microsatellite instability was found in 39% of the samples in the MLL locus, 39% in the APC, 46% in the P16, 23% in the hMLH1 and none in the hMSH2. This study demonstrates that microsatellite instability has more prominent role in pathogenesis of gastric MALT lymphoma than reported to date. We suggest that microsatellite instability should be analyzed with markers adjacent to chromosomal loci that are involved in lymphomas. Our findings support the ‘Real Common Target genes’ theory of high rate of microsatellite instability in specific genes, which are associated with related tumors. |
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This may be explained mainly by arbitrary selection of the tested loci, the use of various techniques of microsatellite instability analysis and by different definitions of replication error positive phenotype. The aim of our study was to evaluate the instability at selected microsatellite markers using the GeneScan Analysis Software. DNA from paraffin-embedded tissue blocks of 13 previously untreated patients with localized gastric MALT lymphoma was extracted. Five microsatellite markers, which are located in hMSH2, hMLH1, P16, APC and MLL loci, were selected from the genetic database. We found genetic instability in tumors of 9/13 patients with gastric MALT lymphoma (69%). Seven of them had replication-error-positive phenotype (54%). Microsatellite instability was found in 39% of the samples in the MLL locus, 39% in the APC, 46% in the P16, 23% in the hMLH1 and none in the hMSH2. This study demonstrates that microsatellite instability has more prominent role in pathogenesis of gastric MALT lymphoma than reported to date. We suggest that microsatellite instability should be analyzed with markers adjacent to chromosomal loci that are involved in lymphomas. 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This may be explained mainly by arbitrary selection of the tested loci, the use of various techniques of microsatellite instability analysis and by different definitions of replication error positive phenotype. The aim of our study was to evaluate the instability at selected microsatellite markers using the GeneScan Analysis Software. DNA from paraffin-embedded tissue blocks of 13 previously untreated patients with localized gastric MALT lymphoma was extracted. Five microsatellite markers, which are located in hMSH2, hMLH1, P16, APC and MLL loci, were selected from the genetic database. We found genetic instability in tumors of 9/13 patients with gastric MALT lymphoma (69%). Seven of them had replication-error-positive phenotype (54%). Microsatellite instability was found in 39% of the samples in the MLL locus, 39% in the APC, 46% in the P16, 23% in the hMLH1 and none in the hMSH2. This study demonstrates that microsatellite instability has more prominent role in pathogenesis of gastric MALT lymphoma than reported to date. We suggest that microsatellite instability should be analyzed with markers adjacent to chromosomal loci that are involved in lymphomas. Our findings support the ‘Real Common Target genes’ theory of high rate of microsatellite instability in specific genes, which are associated with related tumors.</description><subject>Adult</subject><subject>Aged</subject><subject>DNA repair</subject><subject>DNA Repair Enzymes - genetics</subject><subject>Enzymes</subject><subject>Female</subject><subject>genetic instability</subject><subject>Genomes</subject><subject>Genomic Instability</subject><subject>Genotype & phenotype</subject><subject>Humans</subject><subject>Laboratory Medicine</subject><subject>Loss of Heterozygosity</subject><subject>Lymphoma</subject><subject>Lymphoma, B-Cell, Marginal Zone - genetics</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>microsatellite instability</subject><subject>Microsatellite Repeats - genetics</subject><subject>Middle Aged</subject><subject>mucosa-associated lymphoid tissue lymphoma</subject><subject>Mutation</subject><subject>original-article</subject><subject>Pathogenesis</subject><subject>Pathology</subject><subject>Polymerase Chain Reaction</subject><subject>replication-error-positive phenotype</subject><subject>Stomach Neoplasms - genetics</subject><subject>Tumors</subject><subject>Yeast</subject><issn>0893-3952</issn><issn>1530-0285</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNp9kM1rGzEQxUVJaZy0f0AOCaaH3DaVRtKulpxM6Bc49JKexaw8myjshyNpA_7vK7Mmhh5y0sB776eZx9iF4DeCS_OtHzdbTE9jdyMN58CrD2whtOQFB6NP2IKbWhay1nDKzmJ85lwobeATOxUaKoBKL5i89y6MERN1nU-09ENM2Pg87_K8fMSYgnfL-9X6Ydnt-u3T2ONn9rHFLtKXw3vO_v74_nD3q1j_-fn7brUunDIqFahb02ilBPG2qVEhNBzRmMopaJ2rahROICpuVFvVIDXUQFAKkNSSAiPP2fXM3YbxZaKYbO-jy4viQOMUbVnWOVbujV__Mz6PUxjybhYg85QqRTaJ2bS_NwZq7Tb4HsPOCm73ddq3Ou2hzpy5OoCnpqfNMXHoLxtgNsQsDY8Ujj-_R72cQwOmKdAb9ajfzjrlcl99hkbnaXC08YFcspvRv0P_B7Q7oP8</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>Niv, Eva</creator><creator>Bomstein, Yonit</creator><creator>Bernheim, Joelle</creator><creator>Lishner, Michael</creator><general>Elsevier Inc</general><general>Nature Publishing Group US</general><general>Elsevier Limited</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20041101</creationdate><title>Microsatellite instability in gastric MALT lymphoma</title><author>Niv, Eva ; 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This may be explained mainly by arbitrary selection of the tested loci, the use of various techniques of microsatellite instability analysis and by different definitions of replication error positive phenotype. The aim of our study was to evaluate the instability at selected microsatellite markers using the GeneScan Analysis Software. DNA from paraffin-embedded tissue blocks of 13 previously untreated patients with localized gastric MALT lymphoma was extracted. Five microsatellite markers, which are located in hMSH2, hMLH1, P16, APC and MLL loci, were selected from the genetic database. We found genetic instability in tumors of 9/13 patients with gastric MALT lymphoma (69%). Seven of them had replication-error-positive phenotype (54%). Microsatellite instability was found in 39% of the samples in the MLL locus, 39% in the APC, 46% in the P16, 23% in the hMLH1 and none in the hMSH2. This study demonstrates that microsatellite instability has more prominent role in pathogenesis of gastric MALT lymphoma than reported to date. We suggest that microsatellite instability should be analyzed with markers adjacent to chromosomal loci that are involved in lymphomas. Our findings support the ‘Real Common Target genes’ theory of high rate of microsatellite instability in specific genes, which are associated with related tumors.</abstract><cop>New York</cop><pub>Elsevier Inc</pub><pmid>15272275</pmid><doi>10.1038/modpathol.3800207</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Adult Aged DNA repair DNA Repair Enzymes - genetics Enzymes Female genetic instability Genomes Genomic Instability Genotype & phenotype Humans Laboratory Medicine Loss of Heterozygosity Lymphoma Lymphoma, B-Cell, Marginal Zone - genetics Male Medicine Medicine & Public Health microsatellite instability Microsatellite Repeats - genetics Middle Aged mucosa-associated lymphoid tissue lymphoma Mutation original-article Pathogenesis Pathology Polymerase Chain Reaction replication-error-positive phenotype Stomach Neoplasms - genetics Tumors Yeast |
title | Microsatellite instability in gastric MALT lymphoma |
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