Computerized image analysis vs semiquantitative scoring in evaluation of kidney allograft fibrosis and prognosis

Background. Chronic morphological changes in the kidney allograft predict long-term graft function, but there are few studies comparing different methods in assessing chronic lesions. In the present study, we evaluated allograft cortical interstitial fibrosis, and compared semiquantitative assessmen...

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Published in:Nephrology, dialysis, transplantation dialysis, transplantation, 2004-11, Vol.19 (11), p.2838-2845
Main Authors: Sund, Ståle, Grimm, Paul, Reisæter, Anna Varberg, Hovig, Torstein
Format: Article
Language:English
Subjects:
Adult
Aged
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Azo Compounds
Biological and medical sciences
collagen
Emergency and intensive care: renal failure. Dialysis management
Female
Fibrosis
Humans
image analysis
Image Processing, Computer-Assisted
Intensive care medicine
interstitialfibrosis
kidney allograft prognosis
Kidney Transplantation - pathology
Kidney Tubules - pathology
Kidneys
Male
Medical sciences
Middle Aged
Nephrology. Urinary tract diseases
Prognosis
protocol biopsies
Sirius red
Staining and Labeling
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Surgery of the urinary system
Transplantation, Homologous
Tumors of the urinary system
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Summary:Background. Chronic morphological changes in the kidney allograft predict long-term graft function, but there are few studies comparing different methods in assessing chronic lesions. In the present study, we evaluated allograft cortical interstitial fibrosis, and compared semiquantitative assessment with computerized image analysis of Sirius red-stained collagen in prediction of graft prognosis. Methods. Sections were obtained from a series of 1-year protocol living donor kidney graft biopsies (n = 33) and their corresponding baseline specimens (n = 32). At light microscopy, the biopsies were scored for interstitial fibrosis as a percentage of involved tubulointerstitium according to the Banff schema. Quantitation of cortical fractional interstitial fibrosis volume (Vint) was performed with computerized image analysis on coded sections stained with Sirius red. The results were correlated with kidney function at 8–10 years after transplantation, and with late graft loss. Results. There was a significant correlation between the semiquantitative and quantitative methods for measuring cortical interstitial fibrosis in all the biopsies (n = 65, percentage area vs Vint: R = 0.439, P = 0.0003). The correlation further improved when analysing the baseline specimens separately (n = 32, R = 0.704, P
ISSN:0931-0509
1460-2385
1460-2385
DOI:10.1093/ndt/gfh490