Rapid determination of minoxidil in human plasma using ion-pair HPLC

• Published in: Journal of pharmaceutical and biomedical analysis 2004-10, Vol.36 (2), p.377-379
• Main Authors: Zarghi, A., Shafaati, A., Foroutan, S.M., Khoddam, A.
• Format: Article
• Language: English
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Chromatography, High Pressure Liquid; Drug Stability; Fundamental and applied biological sciences. Psychology; General pharmacology; HPLC; Humans; Medical sciences; Minoxidil; Minoxidil - blood; Minoxidil - pharmacokinetics; Pharmacology. Drug treatments; Plasma; Reference Standards; Reproducibility of Results; Solutions; Spectrophotometry, Ultraviolet; Vasodilator Agents - blood; Vasodilator Agents - pharmacokinetics
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2004.06.007

A rapid, simple and sensitive ion-pair high-performance liquid chromatography (HPLC) method has been developed for quantification of minoxidil in plasma. The assay enables the measurement of minoxidil for therapeutic drug monitoring with a minimum detectable limit of 0.5 ng ml −1. The method involves simple, one-step extraction procedure and analytical recovery was complete. The separation was performed on an analytical 150 × 4.6 mm i.d. μbondapak C 18 column. The wavelength was set at 281 nm. The mobile phase was a mixture of 0.01 M sodium dihydrogen phosphate buffer and acetonitrile (60:40, v/v) containing 2.5 mM sodium dodecyl sulphate adjusted to pH 3.5 at a flow rate of 1 ml/min. The column temperature was set at 50 °C. The calibration curve was linear over the concentration range 2–100 ng ml −1. The coefficients of variation for inter-day and intra-day assay were found to be less than 8%.