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Activation, Pronuclear Formation, and Development In Vitro of Pig Oocytes Following Intracytoplasmic Injection of Freeze-Dried Spermatozoa
The fertilization of pig oocytes following intracytoplasmic injection of freeze-dried spermatozoa was evaluated. Activation and male pronuclear (MPN) formation were better in oocytes injected with isolated freeze-dried sperm heads than whole freeze-dried spermatozoa, but cleaved embryos were general...
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| Published in: | Biology of reproduction 2004-11, Vol.71 (5), p.1430-1436 |
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| Language: | English |
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| container_end_page | 1436 |
| container_issue | 5 |
| container_start_page | 1430 |
| container_title | Biology of reproduction |
| container_volume | 71 |
| creator | KWON, In-Kiu PARK, Ki-Eun NIWA, Koji |
| description | The fertilization of pig oocytes following intracytoplasmic injection of freeze-dried spermatozoa was evaluated. Activation
and male pronuclear (MPN) formation were better in oocytes injected with isolated freeze-dried sperm heads than whole freeze-dried
spermatozoa, but cleaved embryos were generally difficult to develop to the morula or blastocyst stage. When spermatozoa were
freeze-dried for 24 h, oocyte activation and MPN formation in activated oocytes after sperm head injection were inhibited.
Embryo development to the blastocyst stage was only obtained after injecting sperm heads isolated from spermatozoa freeze-dried
for 4 h and stored at 4°C. The proportion of embryos that developed to the blastocyst stage was not increased by the treatment
of injected oocytes with Ca ionophore (5â10 μM). Increasing the sperm storage time did not affect oocyte activation or MPN
formation, but blastocyst development was observed only after 1 mo of storage. These results demonstrate that pig oocytes
can be fertilized with appropriately freeze-dried spermatozoa and that the fertilized oocytes can develop to the blastocyst
stage. |
| doi_str_mv | 10.1095/biolreprod.104.031260 |
| format | article |
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and male pronuclear (MPN) formation were better in oocytes injected with isolated freeze-dried sperm heads than whole freeze-dried
spermatozoa, but cleaved embryos were generally difficult to develop to the morula or blastocyst stage. When spermatozoa were
freeze-dried for 24 h, oocyte activation and MPN formation in activated oocytes after sperm head injection were inhibited.
Embryo development to the blastocyst stage was only obtained after injecting sperm heads isolated from spermatozoa freeze-dried
for 4 h and stored at 4°C. The proportion of embryos that developed to the blastocyst stage was not increased by the treatment
of injected oocytes with Ca ionophore (5â10 μM). Increasing the sperm storage time did not affect oocyte activation or MPN
formation, but blastocyst development was observed only after 1 mo of storage. These results demonstrate that pig oocytes
can be fertilized with appropriately freeze-dried spermatozoa and that the fertilized oocytes can develop to the blastocyst
stage.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod.104.031260</identifier><identifier>PMID: 15215192</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Biological and medical sciences ; Blastocyst - physiology ; Calcimycin - pharmacology ; Cell Culture Techniques ; Cell Nucleus - physiology ; Embryonic Development ; Female ; Freeze Drying ; Fundamental and applied biological sciences. Psychology ; Ionophores - pharmacology ; Male ; Mammalian male genital system ; Morphology. Physiology ; Oocytes - drug effects ; Oocytes - physiology ; Semen Preservation ; Sperm Head ; Sperm Injections, Intracytoplasmic ; Spermatozoa ; Swine ; Temperature ; Time Factors ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 2004-11, Vol.71 (5), p.1430-1436</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16219109$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15215192$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>KWON, In-Kiu</creatorcontrib><creatorcontrib>PARK, Ki-Eun</creatorcontrib><creatorcontrib>NIWA, Koji</creatorcontrib><title>Activation, Pronuclear Formation, and Development In Vitro of Pig Oocytes Following Intracytoplasmic Injection of Freeze-Dried Spermatozoa</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>The fertilization of pig oocytes following intracytoplasmic injection of freeze-dried spermatozoa was evaluated. Activation
and male pronuclear (MPN) formation were better in oocytes injected with isolated freeze-dried sperm heads than whole freeze-dried
spermatozoa, but cleaved embryos were generally difficult to develop to the morula or blastocyst stage. When spermatozoa were
freeze-dried for 24 h, oocyte activation and MPN formation in activated oocytes after sperm head injection were inhibited.
Embryo development to the blastocyst stage was only obtained after injecting sperm heads isolated from spermatozoa freeze-dried
for 4 h and stored at 4°C. The proportion of embryos that developed to the blastocyst stage was not increased by the treatment
of injected oocytes with Ca ionophore (5â10 μM). Increasing the sperm storage time did not affect oocyte activation or MPN
formation, but blastocyst development was observed only after 1 mo of storage. These results demonstrate that pig oocytes
can be fertilized with appropriately freeze-dried spermatozoa and that the fertilized oocytes can develop to the blastocyst
stage.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blastocyst - physiology</subject><subject>Calcimycin - pharmacology</subject><subject>Cell Culture Techniques</subject><subject>Cell Nucleus - physiology</subject><subject>Embryonic Development</subject><subject>Female</subject><subject>Freeze Drying</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Ionophores - pharmacology</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Morphology. Physiology</subject><subject>Oocytes - drug effects</subject><subject>Oocytes - physiology</subject><subject>Semen Preservation</subject><subject>Sperm Head</subject><subject>Sperm Injections, Intracytoplasmic</subject><subject>Spermatozoa</subject><subject>Swine</subject><subject>Temperature</subject><subject>Time Factors</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNpFkcFu1DAQhi0EokvhEUC-0BMpHjvxOseqZaFSpVYq4hpNnMmuKycOdrZR-wg8NV6xqKfR_PrmH838jH0EcQ6irr62LvhIUwxd7stzoUBq8YqtoJJ1sZbavGYrIYQulNLqhL1L6UEIKJVUb9lJhqCCWq7Ynws7u0ecXRi_8LsYxr31hJFvQhyOKo4dv6JH8mEaaJz59ch_uTkGHnp-57b8NtinmVIe8T4sbtxmYo6YxTB5TIOzWXgge3A7zGwi0TMVV9FRx-8nOiwKzwHfszc9-kQfjvWU3W--_bz8Udzcfr--vLgpdvmquUBVdgZb6nujtMA1ldB2qBH7FsCUIG1rtCiVMQBlrdZKaCtB15qMFEKdsrN_rvl1v_eU5mZwyZL3OFLYp0bruq4MyAx-OoL7dqCumaIbMD41_3-Xgc9HAJNF30ccrUsvnJZQ56heNu7cdre4SE0a0Ptsq5plWdbQVE0ORqi_RXePcQ</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>KWON, In-Kiu</creator><creator>PARK, Ki-Eun</creator><creator>NIWA, Koji</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20041101</creationdate><title>Activation, Pronuclear Formation, and Development In Vitro of Pig Oocytes Following Intracytoplasmic Injection of Freeze-Dried Spermatozoa</title><author>KWON, In-Kiu ; PARK, Ki-Eun ; NIWA, Koji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h268t-a34d8abeff8360a7e41bda6aafb118412cb8604388114937306c21696e82003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blastocyst - physiology</topic><topic>Calcimycin - pharmacology</topic><topic>Cell Culture Techniques</topic><topic>Cell Nucleus - physiology</topic><topic>Embryonic Development</topic><topic>Female</topic><topic>Freeze Drying</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Ionophores - pharmacology</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Morphology. Physiology</topic><topic>Oocytes - drug effects</topic><topic>Oocytes - physiology</topic><topic>Semen Preservation</topic><topic>Sperm Head</topic><topic>Sperm Injections, Intracytoplasmic</topic><topic>Spermatozoa</topic><topic>Swine</topic><topic>Temperature</topic><topic>Time Factors</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KWON, In-Kiu</creatorcontrib><creatorcontrib>PARK, Ki-Eun</creatorcontrib><creatorcontrib>NIWA, Koji</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>KWON, In-Kiu</au><au>PARK, Ki-Eun</au><au>NIWA, Koji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Activation, Pronuclear Formation, and Development In Vitro of Pig Oocytes Following Intracytoplasmic Injection of Freeze-Dried Spermatozoa</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2004-11-01</date><risdate>2004</risdate><volume>71</volume><issue>5</issue><spage>1430</spage><epage>1436</epage><pages>1430-1436</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>The fertilization of pig oocytes following intracytoplasmic injection of freeze-dried spermatozoa was evaluated. Activation
and male pronuclear (MPN) formation were better in oocytes injected with isolated freeze-dried sperm heads than whole freeze-dried
spermatozoa, but cleaved embryos were generally difficult to develop to the morula or blastocyst stage. When spermatozoa were
freeze-dried for 24 h, oocyte activation and MPN formation in activated oocytes after sperm head injection were inhibited.
Embryo development to the blastocyst stage was only obtained after injecting sperm heads isolated from spermatozoa freeze-dried
for 4 h and stored at 4°C. The proportion of embryos that developed to the blastocyst stage was not increased by the treatment
of injected oocytes with Ca ionophore (5â10 μM). Increasing the sperm storage time did not affect oocyte activation or MPN
formation, but blastocyst development was observed only after 1 mo of storage. These results demonstrate that pig oocytes
can be fertilized with appropriately freeze-dried spermatozoa and that the fertilized oocytes can develop to the blastocyst
stage.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>15215192</pmid><doi>10.1095/biolreprod.104.031260</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Biology of reproduction, 2004-11, Vol.71 (5), p.1430-1436 |
| issn | 0006-3363 1529-7268 |
| language | eng |
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| source | Oxford Journals Online |
| subjects | Animals Biological and medical sciences Blastocyst - physiology Calcimycin - pharmacology Cell Culture Techniques Cell Nucleus - physiology Embryonic Development Female Freeze Drying Fundamental and applied biological sciences. Psychology Ionophores - pharmacology Male Mammalian male genital system Morphology. Physiology Oocytes - drug effects Oocytes - physiology Semen Preservation Sperm Head Sperm Injections, Intracytoplasmic Spermatozoa Swine Temperature Time Factors Vertebrates: reproduction |
| title | Activation, Pronuclear Formation, and Development In Vitro of Pig Oocytes Following Intracytoplasmic Injection of Freeze-Dried Spermatozoa |
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