Crystallization and preliminary X-ray crystallographic studies of human 2′,3′-cyclic nucleotide 3′-phosphodiesterase

The catalytic fragment of human 2′,3′‐cyclic nucleotide 3′‐phosphodiesterase (hCNP‐CF) has been crystallized by the hanging‐drop vapour‐diffusion method using polyethylene glycol 300 as the precipitating agent. The crystals belong to the orthorhombic space group P212121, with unit‐cell parameters a...

Full description

Saved in:
Bibliographic Details
Published in:Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2004-11, Vol.60 (11), p.2095-2097
Main Authors: Sakamoto, Yasumitsu, Tanaka, Nobutada, Ichimiya, Tomomi, Kurihara, Tadashi, Nakamura, Kazuo T.
Format: Article
Language:English
Subjects:
2',3'-Cyclic Nucleotide 3'-Phosphodiesterase
2H phosphoesterase superfamily
central nervous system
Crystallization
Crystallography, X-Ray
Humans
myelin
phosphodiesterase
Phosphoric Diester Hydrolases - chemistry
Phosphoric Diester Hydrolases - genetics
Phosphoric Diester Hydrolases - isolation & purification
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The catalytic fragment of human 2′,3′‐cyclic nucleotide 3′‐phosphodiesterase (hCNP‐CF) has been crystallized by the hanging‐drop vapour‐diffusion method using polyethylene glycol 300 as the precipitating agent. The crystals belong to the orthorhombic space group P212121, with unit‐cell parameters a = 44.39, b = 55.35, c = 78.76 Å. There is one molecule per asymmetric unit. The crystals diffract to at least 1.8 Å resolution using synchrotron radiation and are suitable for X‐ray structure analysis at high resolution.
ISSN:1399-0047
0907-4449
1399-0047
DOI:10.1107/S0907444904024126