Physicochemical factors influencing bacterial transfer from contact lenses to surfaces with different roughness and wettability

The aim of this study was to determine the transfer of Pseudomonas aeruginosa No. 3 and Staphylococcus aureus 835 from contact lenses to surfaces with different hydrophobicity and roughness. Bacteria were allowed to adhere to contact lenses (Surevue, PureVision, or Focus Night & Day) by incubati...

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Bibliographic Details
Published in:Journal of biomedical materials research 2004-11, Vol.71B (2), p.336-342
Main Authors: Vermeltfoort, Pit B. J., van der Mei, Henny C., Busscher, Henk J., Hooymans, Johanna M. M., Bruinsma, Gerda M.
Format: Article
Language:English
Subjects:
Bacteria - chemistry
bacterial transfer
Chemical Phenomena
Chemistry, Physical
contact lens
Contact Lenses - microbiology
Glass
hydrophobicity
Microscopy, Atomic Force
Polymethyl Methacrylate
Pseudomonas aeruginosa
Pseudomonas aeruginosa - chemistry
roughness
Silicone Elastomers
Staphylococcus aureus
Staphylococcus aureus - chemistry
substratum surfaces
Surface Properties
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Summary:The aim of this study was to determine the transfer of Pseudomonas aeruginosa No. 3 and Staphylococcus aureus 835 from contact lenses to surfaces with different hydrophobicity and roughness. Bacteria were allowed to adhere to contact lenses (Surevue, PureVision, or Focus Night & Day) by incubating the lenses in a bacterial suspension for 30 min. The contaminated lenses were put on a glass, poly(methylmethacrylate), or silicone rubber substratum, shaped to mimic the eye. After 2 and 16 h, lenses were separated from the substrata and bacteria were swabbed off from the respective surfaces and resuspended in saline. Appropriate serial dilutions of these suspensions were made, from which aliquots were plated on agar for enumeration. Bacterial transfer varied between 4 and 60%, depending on the combination of strain, contact time, contact lens, and substratum surface. For P. aeruginosa No. 3, transfer was significantly higher after 16 h than after 2 h, whereas less increase with time was seen for S. aureus 835. Bacterial transfer from all tested contact lenses was least to silicone rubber, the most hydrophobic and roughest substratum surface included. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 71B: 336–342, 2004
ISSN:1552-4973
0021-9304
1552-4981
DOI:10.1002/jbm.b.30100