Validation of a New Automated Renin Assay

Measurement of plasma renin is important for the treatment of patients with congenital adrenal hyperplasia (CAH) and in the evaluation of patients with suspected hyperaldosteronism. Immunologic assays for plasma renin offer easier implementation and standardization than enzyme-kinetic assays for pla...

Full description

Saved in:
Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 2004-11, Vol.50 (11), p.2111-2116
Main Authors: de Bruin, Rene A, Bouhuizen, Angelique, Diederich, Sven, Perschel, Frank H, Boomsma, Frans, Deinum, Jaap
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Analytical, structural and metabolic biochemistry
Autoanalysis
Biological and medical sciences
Centrifugation
Child
Child, Preschool
Female
Fundamental and applied biological sciences. Psychology
Humans
Hypertension
Immunoassay - methods
Investigative techniques, diagnostic techniques (general aspects)
Luminescent Measurements
Male
Medical sciences
Middle Aged
Plasma
Renin - blood
Sensitivity and Specificity
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Measurement of plasma renin is important for the treatment of patients with congenital adrenal hyperplasia (CAH) and in the evaluation of patients with suspected hyperaldosteronism. Immunologic assays for plasma renin offer easier implementation and standardization than enzyme-kinetic assays for plasma renin activity, but their sensitivity and specificity have been questioned. We studied a renin immunochemiluminescence assay on an automated platform. Renin was measured by an enzymatic assay, by IRMA, and by the new Nichols Advantage Specialty System immunochemiluminometric assay (ICMA), in plasmas from unselected individuals from our outpatient departments and in samples from patients with selected diagnoses. The detection limit in the ICMA was 0.1 mU/L. The recovery was >90%, and the imprecision (CV) was generally
ISSN:0009-9147
1530-8561
DOI:10.1373/clinchem.2004.032052