Ageing affects the differentiation potential of human myoblasts

The ageing process causes a reduction in the regenerative potential of skeletal muscles eventually leading to diminished muscle strength. In this work we investigated if ageing affects the excitation–contraction coupling mechanism in human myotubes derived from human satellite cells, thereby contrib...

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Bibliographic Details
Published in:Experimental gerontology 2004-10, Vol.39 (10), p.1545-1554
Main Authors: Lorenzon, Paola, Bandi, Elena, de Guarrini, Francesca, Pietrangelo, Tiziana, Schäfer, Ralf, Zweyer, Margit, Wernig, Anton, Ruzzier, Fabio
Format: Article
Language:English
Subjects:
Adult
Aged
Ageing
Aging - physiology
Calcium Channels - physiology
Cell culture
Cell Differentiation - physiology
Cell Division - physiology
Cell Fusion
Cells, Cultured
Cellular Senescence - physiology
Child, Preschool
Desmin - metabolism
Excitation–contraction coupling mechanism
Human myoblasts
Human satellite cells
Humans
Middle Aged
Muscle Contraction - physiology
Ryanodine receptor channels
Sarcopenia
Satellite Cells, Skeletal Muscle - cytology
Satellite Cells, Skeletal Muscle - metabolism
Satellite Cells, Skeletal Muscle - physiology
Videomicroscopy
Voltage-dependent calcium channels
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Summary:The ageing process causes a reduction in the regenerative potential of skeletal muscles eventually leading to diminished muscle strength. In this work we investigated if ageing affects the excitation–contraction coupling mechanism in human myotubes derived from human satellite cells, thereby contributing to the loss in muscle strength in the aged. To test this hypothesis, satellite cells from differently aged donors were differentiated in vitro and the maturation of the excitation–contraction mechanism was followed by the videoimaging technique monitoring the efficiency of such a mechanism in generating intracellular calcium transients. Our experiments showed a delay in the establishment of the excitation–contraction coupling mechanism depending on the age of the donor. Remarkably, the effect was reproducible in human satellite cells from a young donor aged in vitro, suggesting that the delayed functional maturation was strictly dependent on the number of satellite cell divisions and independent from the host environment.
ISSN:0531-5565
1873-6815
DOI:10.1016/j.exger.2004.07.008