Angiotensin II facilitates autoregulation in the perfused mouse kidney: An optimized in vitro model for assessment of renal vascular and tubular function

Background and Aims:  We have optimized the isolated perfused mouse kidney (IPMK) model for studying renal vascular and tubular function in vitro using 24–28 g C57BL6J mice; the wild type controls for many transgenic mice. Methods and Results:  Buffer composition was optimized for bovine serum album...

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Published in:Nephrology (Carlton, Vic.) Vic.), 2004-10, Vol.9 (5), p.288-296
Main Authors: RAHGOZAR, MAJID, GUAN, ZHENGRONG, MATTHIAS, ANITA, GOBÉ, GLENDA C, ENDRE, ZOLTÁN H
Format: Article
Language:English
Subjects:
angiotensin II
Angiotensin II - physiology
Animals
autoregulation
erythrocytes
Glomerular Filtration Rate
Homeostasis
In Vitro Techniques
Kidney - blood supply
Kidney - physiology
Kidney Tubules - physiology
Male
medullary hypoxia
methacholine
Mice
Mice, Inbred C57BL
mouse kidney
Perfusion
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Summary:Background and Aims:  We have optimized the isolated perfused mouse kidney (IPMK) model for studying renal vascular and tubular function in vitro using 24–28 g C57BL6J mice; the wild type controls for many transgenic mice. Methods and Results:  Buffer composition was optimized for bovine serum albumin concentration (BSA). The effect of adding erythrocytes on renal function and morphology was assessed. Autoregulation was investigated during stepped increases in perfusion pressure. Perfusion for 60 min at 90–110 mmHg with Krebs bicarbonate buffer containing 5.5% BSA, and amino acids produced functional parameters within the in vivo range. Erythrocytes increased renal vascular resistance (3.8 ± 0.2 vs 2.4 ± 0.1 mL/min.mmHg, P 
ISSN:1320-5358
1440-1797
DOI:10.1111/j.1440-1797.2004.00316.x