Expressed gene sequence of bovine IL23A and IL23R

• Published in: Veterinary immunology and immunopathology 2009-04, Vol.128 (4), p.425-430
• Main Authors: Chen, Chuang, Herzig, Carolyn T.A., Baldwin, Cynthia L.
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Base Sequence; cattle; Cattle - genetics; Cattle - immunology; Cloning, Molecular; complementary DNA; exons; genome; genomics; IL23A; IL23R; immune system; interleukin-12; Interleukin-12 - biosynthesis; Interleukin-12 - genetics; Interleukin-12 - immunology; interleukin-17; interleukin-23; Interleukin-23 Subunit p19 - biosynthesis; Interleukin-23 Subunit p19 - genetics; Interleukin-23 Subunit p19 - immunology; interleukins; molecular cloning; molecular genetics; Molecular Sequence Data; nucleotide sequences; Phylogeny; receptors; Receptors, Interleukin - biosynthesis; Receptors, Interleukin - genetics; Receptors, Interleukin - immunology; Reverse Transcriptase Polymerase Chain Reaction - veterinary; RNA; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; Ruminant; Sequence Alignment; T cells; T-lymphocytes; Th17; viability
• ISSN: 0165-2427; 1873-2534
• DOI: 10.1016/j.vetimm.2008.11.021

The cloning and characterization of bovine IL23A and IL23 receptor cDNA from total RNA of PBMC and the genomic organization of the coding sequences are reported. The IL23A partial coding region was found to be 578 nucleotides coded for in 4 exons and shared 84% and 76% identity with human and mouse sequences, respectively. The IL23R complete coding region had 1890 nucleotides coded for in 10 exons and shared 87% and 73% homology with the human and mouse sequences, respectively. Both bovine sequences were more closely related to the human sequences than were mouse sequence. This work was done as part of the U.S. Veterinary Immune Reagent Network whose goal is to develop reagents for investigating diseases in livestock species, poultry and fish.