The use of two-dimension electrophoresis to identify serum biomarkers from patients with dengue haemorrhagic fever

Dengue infection is a major public health problem affecting millions of people living in tropical countries. With no suitable vaccines and specific antiviral drugs, treatment for dengue is usually symptomatic and supportive. Early diagnosis and recognition of severe disease is therefore crucial for...

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Bibliographic Details
Published in:Transactions of the Royal Society of Tropical Medicine and Hygiene 2009-04, Vol.103 (4), p.413-419
Main Authors: Thayan, Ravindran, Huat, Tan Lian, See, Lucy Lum Chai, Tan, Christina Phoay Lay, Khairullah, Nor Shahidah, Yusof, Rohana, Devi, Shamala
Format: Article
Language:English
Subjects:
Adolescent
Adult
alpha 1-Antitrypsin - blood
alpha 1-Antitrypsin - immunology
Arboviral haemorrhagic fevers
Arboviroses
Biological and medical sciences
Biomarkers - blood
Dengue fever
Dengue fevers
Dengue haemorrhagic fever
Dengue Virus - immunology
Disease indicators
Electrophoresis, Gel, Two-Dimensional
Enzyme-Linked Immunosorbent Assay - methods
Female
General aspects
Human viral diseases
Humans
Immunoglobulin M - blood
Infectious diseases
Male
Medical sciences
NS1 protein
Protein marker
RNA, Viral - blood
Severe Dengue - diagnosis
Severe Dengue - immunology
Tropical viral diseases
Viral diseases
Viral Nonstructural Proteins - blood
Viral Nonstructural Proteins - immunology
Young Adult
α1-antitrypsin
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Summary:Dengue infection is a major public health problem affecting millions of people living in tropical countries. With no suitable vaccines and specific antiviral drugs, treatment for dengue is usually symptomatic and supportive. Early diagnosis and recognition of severe disease is therefore crucial for better management of the patient. Two-dimension electrophoresis was used to identify disease-associated proteins that can be used for diagnosis and as drug targets for treatment. Two markers, identified by mass spectrometry analysis as α1-antitrypsin and NS1 proteins were found to be upregulated in dengue fever (DF; n = 10) and dengue haemorrhagic fever (DHF; n = 10) patients compared with healthy individuals ( n = 8). Both α1-antitrypsin and NS1 proteins were overexpressed two-fold in DHF patients compared with DF patients. Our study suggests that α1-antitrypsin and NS1 protein could be used as biomarkers as early indicators of DHF risk among patients with suspected dengue infection.
ISSN:0035-9203
1878-3503
DOI:10.1016/j.trstmh.2008.12.018