A novel dengue vaccine candidate that induces cross-neutralizing antibodies and memory immunity

• Published in: Microbes and infection 2009-02, Vol.11 (2), p.288-295
• Main Authors: Leng, Chih-Hsiang, Liu, Shih-Jen, Tsai, Jy-Ping, Li, Yi-Shiuan, Chen, Mei-Yu, Liu, Hsueh-Hung, Lien, Shu-Pei, Yueh, Andrew, Hsiao, Kuang-Nan, Lai, Li-Wei, Liu, Feng-Chuan, Chong, Pele, Chen, Hsin-Wei
• Format: Article
• Language: English
• Subjects: Adjuvants, Immunologic - administration & dosage; Adjuvants, Immunologic - pharmacology; Aluminum Compounds - administration & dosage; Aluminum Compounds - pharmacology; Amino Acid Sequence; Animals; Antibodies, Viral - blood; Applied microbiology; Arboviroses; Biological and medical sciences; Consensus Sequence; Dengue fevers; Dengue Vaccines - genetics; Dengue Vaccines - immunology; Dengue virus; Dengue Virus - genetics; Dengue Virus - immunology; Envelope protein; Fundamental and applied biological sciences. Psychology; Human viral diseases; Immunologic Memory; Infectious diseases; Medical sciences; Mice; Mice, Inbred BALB C; Microbiology; Miscellaneous; Molecular Sequence Data; Neutralization Tests; Neutralizing antibody; Phosphates - administration & dosage; Phosphates - pharmacology; Sequence Alignment; Subunit vaccine; Tropical viral diseases; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccines, Synthetic - genetics; Vaccines, Synthetic - immunology; Viral diseases; Viral Envelope Proteins - genetics; Viral Envelope Proteins - immunology; Virology
• ISSN: 1286-4579; 1769-714X
• DOI: 10.1016/j.micinf.2008.12.004

A novel dengue vaccine candidate comprised of a consensus dengue virus envelope protein domain III (cED III) was developed to fight against dengue virus infection. The amino acid sequence of this novel cED III was obtained by alignment of amino acid sequences from different isolates of the four serotypes of dengue viruses. A proof-of-concept study demonstrated that BALB/c mice immunized with the recombinant cED III developed neutralizing antibodies against all serotypes of dengue virus. Moreover, formulation of recombinant cED III with aluminum phosphate could induce long-lasting antibody responses and anamnestic neutralizing antibody responses following challenge with dengue virus at week 28 after priming. These results demonstrate the possibility of developing a single tetravalent vaccine against dengue viral infections.