Profiling analysis of oligosaccharides in antibody pharmaceuticals by capillary electrophoresis

Carbohydrate chains in glycoprotein pharmaceuticals have important roles for the expression of their biological activities. Therefore, development of an assessment method for the carbohydrate chains is an important parameter for quality control of glycoprotein pharmaceuticals such as newly developed...

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Bibliographic Details
Published in:Journal of Chromatography A 2004-10, Vol.1050 (2), p.211-216
Main Authors: KAMODA, Satoru, NOMURA, Chie, KINOSHITA, Mitsuhiro, NISHIURA, Saori, ISHIKAWA, Rika, KAKEHI, Kazuaki, KAWASAKI, Nana, HAYAKAWA, Takao
Format: Article
Language:English
Subjects:
Aminobenzoates - analysis
Analysis
Analytical biochemistry: general aspects, technics, instrumentation
Analytical chemistry
Analytical, structural and metabolic biochemistry
Antibodies, Monoclonal - analysis
Antibodies, Monoclonal, Humanized
Antibodies, Monoclonal, Murine-Derived
Antineoplastic Agents - analysis
Applied sciences
Biological and medical sciences
Chemical industry and chemicals
Chemistry
Chromatographic methods and physical methods associated with chromatography
Cosmetics, toiletries
Electrophoresis, Capillary
Exact sciences and technology
Fluorescent Dyes
Fundamental and applied biological sciences. Psychology
General pharmacology
Medical sciences
meta-Aminobenzoates
Oligosaccharides - analysis
Other chromatographic methods
Pharmacology. Drug treatments
Pyrenes - analysis
Rituximab
Trastuzumab
Washing products. Cosmetics and toiletries. Perfumes
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Summary:Carbohydrate chains in glycoprotein pharmaceuticals have important roles for the expression of their biological activities. Therefore, development of an assessment method for the carbohydrate chains is an important parameter for quality control of glycoprotein pharmaceuticals such as newly developed therapeutic antibodies. In this report, we applied capillary electrophoresis with laser-induced fluorescence detection to the analysis of carbohydrate chains after releasing with glycoamidase followed by derivatization with 3-aminobenzoic acid. We found that four major oligosaccharides present in antibody pharmaceuticals were successfully separated with good resolution. The present method showed good precision in both migration times and relative peak areas, and gave comparable accuracy with that using a derivatization method with 8-aminopyrene-1,3,6-trisulfonate.
ISSN:0021-9673
DOI:10.1016/s0021-9673(04)01399-8