Mls presentation by peritoneal cavity B cells

DBA/2J spleen and peritoneal cells were compared for their ability to present the minor lymphocyte stimulatory superantigen Mls-1a. Although capable of Mls presentation in vivo, peritoneal cells were less effective than spleen cells in vitro. This difference was not due to cell concentration or cult...

Full description

Saved in:
Bibliographic Details
Published in:Immunobiology (1979) 2004-01, Vol.209 (3), p.255-264
Main Authors: Riggs, James E., Howell, Koko F., Taylor, Justin, Mahjied, Tazee, Prokopenko, Nataliya, Alvarez, John, Coleman, Clenton
Format: Article
Language:English
Subjects:
Animals
Antigen Presentation - immunology
B cells
B-Lymphocytes - immunology
Female
Male
Mice
Mice, Inbred Strains
Minor Lymphocyte Stimulatory Antigens - immunology
Minor Lymphocyte Stimulatory Antigens - metabolism
Mls
Peritoneal Cavity - cytology
Spleen - cytology
Spleen - immunology
Superantigens
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:DBA/2J spleen and peritoneal cells were compared for their ability to present the minor lymphocyte stimulatory superantigen Mls-1a. Although capable of Mls presentation in vivo, peritoneal cells were less effective than spleen cells in vitro. This difference was not due to cell concentration or culture duration. Flow cytometric comparison of spleen and peritoneal B cells revealed no significant differences in cell surface markers needed for cognate interaction with T cells. Resolution of peritoneal B cell subsets by cell sorting revealed that even though B-1 cells were capable of Mls presentation, they were less effective than B-2 cells. Mixing experiments showed that B-1 cells did not inhibit B-2 cell presentation of Mls. In contrast, total peritoneal cells inhibited T cell responses to Mls presented by spleen cells. The peritoneal cavity harbors B cells that can present Mls as well as other cells that can suppress this response.
ISSN:0171-2985
1878-3279
DOI:10.1016/j.imbio.2004.03.008