Expression of Estrogen Receptor-Beta Isoforms in Barrett's Metaplasia, Dysplasia and Esophageal Adenocarcinoma

• Published in: Anticancer research 2004-09, Vol.24 (5A), p.2919-2924
• Main Authors: LIANG LIU, CHIRALA, Minni, YOUNES, Mamoun
• Format: Article
• Language: English
• Subjects: Adenocarcinoma - metabolism; Barrett Esophagus - metabolism; Barrett Esophagus - pathology; Biological and medical sciences; Esophageal Neoplasms - metabolism; Esophagus; Estrogen Receptor beta - biosynthesis; Gastroenterology. Liver. Pancreas. Abdomen; Humans; Immunohistochemistry; Medical sciences; Metaplasia - metabolism; Other diseases. Semiology; Protein Isoforms; Tumors
• ISSN: 0250-7005; 1791-7530

We have previously shown that the majority of esophageal adenocarcinomas (EA), and its precursor Barrett's metaplasia (BM), express estrogen receptor beta (ER-B). Several isoforms of ER-B have been described and are presumed to have different functions, but their distribution in BM and EA is not known. The aim of this work was to determine which ER-B isoforms are expressed in EA and BM. Sections of formalin-fixed and paraffin-embedded esophageal tissue from 33 esophageactomy specimens, of which 27 had invasive EA, were stained for the ERB isoforms ER-B1, ER-B2, ER-B3 and ER-B5 utilizing the immunoperoxidase method. ER-B1 was detected in 23 out of 27 (85%) EA compared to 3 out of 14 (21%) Barrett's metaplasia negative for dysplasia (BMND) (p=0.0001); ER-B2 was expressed in 22 out of 27 (81%) EA in contrast to 3 out of 14 (21%) BMND (p=0.0004); ER-B3 was positive in 27 out of 27 (100%) EA in contrast to only 1 out of 14 (7%) BMND (p