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Expression of Estrogen Receptor-Beta Isoforms in Barrett's Metaplasia, Dysplasia and Esophageal Adenocarcinoma
We have previously shown that the majority of esophageal adenocarcinomas (EA), and its precursor Barrett's metaplasia (BM), express estrogen receptor beta (ER-B). Several isoforms of ER-B have been described and are presumed to have different functions, but their distribution in BM and EA is no...
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| Published in: | Anticancer research 2004-09, Vol.24 (5A), p.2919-2924 |
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| container_title | Anticancer research |
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| creator | LIANG LIU CHIRALA, Minni YOUNES, Mamoun |
| description | We have previously shown that the majority of esophageal adenocarcinomas (EA), and its precursor Barrett's metaplasia (BM),
express estrogen receptor beta (ER-B). Several isoforms of ER-B have been described and are presumed to have different functions,
but their distribution in BM and EA is not known. The aim of this work was to determine which ER-B isoforms are expressed
in EA and BM. Sections of formalin-fixed and paraffin-embedded esophageal tissue from 33 esophageactomy specimens, of which
27 had invasive EA, were stained for the ERB isoforms ER-B1, ER-B2, ER-B3 and ER-B5 utilizing the immunoperoxidase method.
ER-B1 was detected in 23 out of 27 (85%) EA compared to 3 out of 14 (21%) Barrett's metaplasia negative for dysplasia (BMND)
(p=0.0001); ER-B2 was expressed in 22 out of 27 (81%) EA in contrast to 3 out of 14 (21%) BMND (p=0.0004); ER-B3 was positive
in 27 out of 27 (100%) EA in contrast to only 1 out of 14 (7%) BMND (p |
| format | article |
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express estrogen receptor beta (ER-B). Several isoforms of ER-B have been described and are presumed to have different functions,
but their distribution in BM and EA is not known. The aim of this work was to determine which ER-B isoforms are expressed
in EA and BM. Sections of formalin-fixed and paraffin-embedded esophageal tissue from 33 esophageactomy specimens, of which
27 had invasive EA, were stained for the ERB isoforms ER-B1, ER-B2, ER-B3 and ER-B5 utilizing the immunoperoxidase method.
ER-B1 was detected in 23 out of 27 (85%) EA compared to 3 out of 14 (21%) Barrett's metaplasia negative for dysplasia (BMND)
(p=0.0001); ER-B2 was expressed in 22 out of 27 (81%) EA in contrast to 3 out of 14 (21%) BMND (p=0.0004); ER-B3 was positive
in 27 out of 27 (100%) EA in contrast to only 1 out of 14 (7%) BMND (p<0001); ER-B5 was detected in 27 out of 27 (100%) EA
compared to 9 out of 14 (62%) of BMND (p=0.0027). High- and low-grade dysplasia showed a similar ER-beta isoform expression
profile to that of EA. Cancers invasive through the esophageal wall had a higher percent of cells with cytoplasmic expression
of ER-B1 than tumors limited to the wall (T3 vs. T1 and T2, p=0.051). We conclude that ER-B1, ER-B2, ER-B3 and ER-B5 are overexpressed
in EA compared to its precursor lesion BMND, suggesting a significant biological role for steroid hormones in EA.</description><identifier>ISSN: 0250-7005</identifier><identifier>EISSN: 1791-7530</identifier><identifier>PMID: 15517897</identifier><language>eng</language><publisher>Attiki: International Institute of Anticancer Research</publisher><subject>Adenocarcinoma - metabolism ; Barrett Esophagus - metabolism ; Barrett Esophagus - pathology ; Biological and medical sciences ; Esophageal Neoplasms - metabolism ; Esophagus ; Estrogen Receptor beta - biosynthesis ; Gastroenterology. Liver. Pancreas. Abdomen ; Humans ; Immunohistochemistry ; Medical sciences ; Metaplasia - metabolism ; Other diseases. Semiology ; Protein Isoforms ; Tumors</subject><ispartof>Anticancer research, 2004-09, Vol.24 (5A), p.2919-2924</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16196330$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15517897$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LIANG LIU</creatorcontrib><creatorcontrib>CHIRALA, Minni</creatorcontrib><creatorcontrib>YOUNES, Mamoun</creatorcontrib><title>Expression of Estrogen Receptor-Beta Isoforms in Barrett's Metaplasia, Dysplasia and Esophageal Adenocarcinoma</title><title>Anticancer research</title><addtitle>Anticancer Res</addtitle><description>We have previously shown that the majority of esophageal adenocarcinomas (EA), and its precursor Barrett's metaplasia (BM),
express estrogen receptor beta (ER-B). Several isoforms of ER-B have been described and are presumed to have different functions,
but their distribution in BM and EA is not known. The aim of this work was to determine which ER-B isoforms are expressed
in EA and BM. Sections of formalin-fixed and paraffin-embedded esophageal tissue from 33 esophageactomy specimens, of which
27 had invasive EA, were stained for the ERB isoforms ER-B1, ER-B2, ER-B3 and ER-B5 utilizing the immunoperoxidase method.
ER-B1 was detected in 23 out of 27 (85%) EA compared to 3 out of 14 (21%) Barrett's metaplasia negative for dysplasia (BMND)
(p=0.0001); ER-B2 was expressed in 22 out of 27 (81%) EA in contrast to 3 out of 14 (21%) BMND (p=0.0004); ER-B3 was positive
in 27 out of 27 (100%) EA in contrast to only 1 out of 14 (7%) BMND (p<0001); ER-B5 was detected in 27 out of 27 (100%) EA
compared to 9 out of 14 (62%) of BMND (p=0.0027). High- and low-grade dysplasia showed a similar ER-beta isoform expression
profile to that of EA. Cancers invasive through the esophageal wall had a higher percent of cells with cytoplasmic expression
of ER-B1 than tumors limited to the wall (T3 vs. T1 and T2, p=0.051). We conclude that ER-B1, ER-B2, ER-B3 and ER-B5 are overexpressed
in EA compared to its precursor lesion BMND, suggesting a significant biological role for steroid hormones in EA.</description><subject>Adenocarcinoma - metabolism</subject><subject>Barrett Esophagus - metabolism</subject><subject>Barrett Esophagus - pathology</subject><subject>Biological and medical sciences</subject><subject>Esophageal Neoplasms - metabolism</subject><subject>Esophagus</subject><subject>Estrogen Receptor beta - biosynthesis</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Medical sciences</subject><subject>Metaplasia - metabolism</subject><subject>Other diseases. Semiology</subject><subject>Protein Isoforms</subject><subject>Tumors</subject><issn>0250-7005</issn><issn>1791-7530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNpFkFtL5EAQhYMoznj5C9Iv6ouB7qQv6cdRZ3cFF2FZn0OlU5lpSbpjV4Z1_r0BZ_GpDpyP70AdZUthrMiNKvlxtuSF4rnhXC2yM6I3zrW2VXmaLYRSwlTWLLOw_hgTEvkYWOzYmqYUNxjYH3Q4TjHl9zgBe6LYxTQQ84HdQ0o4TbfEfs_V2AN5uGOPe_qKDEI7a-K4hQ1Cz1YthuggOR_iABfZSQc94eXhnmevP9Z_H37lzy8_nx5Wz_m20NWUq6oVojHYANoKO2OM0rK10ihlXFtaoaumMdLJqigkl1JYVSksXCedQYFYnmc3X94xxfcd0lQPnhz2PQSMO6q14YXmSs_g1QHcNQO29Zj8AGlf___QDFwfACAHfZcgOE_fnBZWlyX_Xtz6zfafT1jTAH0_a8saUiFrtaoLK2z5CV3AfYY</recordid><startdate>20040901</startdate><enddate>20040901</enddate><creator>LIANG LIU</creator><creator>CHIRALA, Minni</creator><creator>YOUNES, Mamoun</creator><general>International Institute of Anticancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20040901</creationdate><title>Expression of Estrogen Receptor-Beta Isoforms in Barrett's Metaplasia, Dysplasia and Esophageal Adenocarcinoma</title><author>LIANG LIU ; CHIRALA, Minni ; YOUNES, Mamoun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h268t-58d11b7ebae98ef777564d947557cd39168bb74c4822404419585e2cf4c7e1ee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adenocarcinoma - metabolism</topic><topic>Barrett Esophagus - metabolism</topic><topic>Barrett Esophagus - pathology</topic><topic>Biological and medical sciences</topic><topic>Esophageal Neoplasms - metabolism</topic><topic>Esophagus</topic><topic>Estrogen Receptor beta - biosynthesis</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Medical sciences</topic><topic>Metaplasia - metabolism</topic><topic>Other diseases. Semiology</topic><topic>Protein Isoforms</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LIANG LIU</creatorcontrib><creatorcontrib>CHIRALA, Minni</creatorcontrib><creatorcontrib>YOUNES, Mamoun</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Anticancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LIANG LIU</au><au>CHIRALA, Minni</au><au>YOUNES, Mamoun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of Estrogen Receptor-Beta Isoforms in Barrett's Metaplasia, Dysplasia and Esophageal Adenocarcinoma</atitle><jtitle>Anticancer research</jtitle><addtitle>Anticancer Res</addtitle><date>2004-09-01</date><risdate>2004</risdate><volume>24</volume><issue>5A</issue><spage>2919</spage><epage>2924</epage><pages>2919-2924</pages><issn>0250-7005</issn><eissn>1791-7530</eissn><abstract>We have previously shown that the majority of esophageal adenocarcinomas (EA), and its precursor Barrett's metaplasia (BM),
express estrogen receptor beta (ER-B). Several isoforms of ER-B have been described and are presumed to have different functions,
but their distribution in BM and EA is not known. The aim of this work was to determine which ER-B isoforms are expressed
in EA and BM. Sections of formalin-fixed and paraffin-embedded esophageal tissue from 33 esophageactomy specimens, of which
27 had invasive EA, were stained for the ERB isoforms ER-B1, ER-B2, ER-B3 and ER-B5 utilizing the immunoperoxidase method.
ER-B1 was detected in 23 out of 27 (85%) EA compared to 3 out of 14 (21%) Barrett's metaplasia negative for dysplasia (BMND)
(p=0.0001); ER-B2 was expressed in 22 out of 27 (81%) EA in contrast to 3 out of 14 (21%) BMND (p=0.0004); ER-B3 was positive
in 27 out of 27 (100%) EA in contrast to only 1 out of 14 (7%) BMND (p<0001); ER-B5 was detected in 27 out of 27 (100%) EA
compared to 9 out of 14 (62%) of BMND (p=0.0027). High- and low-grade dysplasia showed a similar ER-beta isoform expression
profile to that of EA. Cancers invasive through the esophageal wall had a higher percent of cells with cytoplasmic expression
of ER-B1 than tumors limited to the wall (T3 vs. T1 and T2, p=0.051). We conclude that ER-B1, ER-B2, ER-B3 and ER-B5 are overexpressed
in EA compared to its precursor lesion BMND, suggesting a significant biological role for steroid hormones in EA.</abstract><cop>Attiki</cop><pub>International Institute of Anticancer Research</pub><pmid>15517897</pmid><tpages>6</tpages></addata></record> |
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| source | EZB Electronic Journals Library |
| subjects | Adenocarcinoma - metabolism Barrett Esophagus - metabolism Barrett Esophagus - pathology Biological and medical sciences Esophageal Neoplasms - metabolism Esophagus Estrogen Receptor beta - biosynthesis Gastroenterology. Liver. Pancreas. Abdomen Humans Immunohistochemistry Medical sciences Metaplasia - metabolism Other diseases. Semiology Protein Isoforms Tumors |
| title | Expression of Estrogen Receptor-Beta Isoforms in Barrett's Metaplasia, Dysplasia and Esophageal Adenocarcinoma |
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