Activation of lignin peroxidase in organic media by reversed micelles

Activation of lignin peroxidase (LIP) in an organic solvent by reversed micelles was investigated. Bis(2-ethylhexyl)sulfosuccinate sodium salt (AOT) was used as a surfactant to form a reversed micelle. Lyophilized LIP from an optimized aqueous solution exhibited no enzymatic activity in any organic...

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Bibliographic Details
Published in:Biotechnology and bioengineering 2004-11, Vol.88 (4), p.495-501
Main Authors: Kimura, M, Michizoe, J, Oakazaki, S, Furusaki, S, Goto, M, Tanaka, H, Wariishi, H
Format: Article
Language:English
Subjects:
biodegradation
Biological and medical sciences
Biotechnology
Colloids - chemistry
Dioctyl Sulfosuccinic Acid - chemistry
Enzyme Activation
Enzyme Stability
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
lignin peroxidase
Micelles
Organic Chemicals - chemistry
Organic chemistry
organic solvent
Peroxidases - chemistry
Phenols - chemistry
reversed micellar system
solvents
Solvents - chemistry
Substrate Specificity
Surface-Active Agents - chemistry
Temperature
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Summary:Activation of lignin peroxidase (LIP) in an organic solvent by reversed micelles was investigated. Bis(2-ethylhexyl)sulfosuccinate sodium salt (AOT) was used as a surfactant to form a reversed micelle. Lyophilized LIP from an optimized aqueous solution exhibited no enzymatic activity in any organic solvents examined in this study; however, LIP was catalytically active by being entrapped in the AOT reversed micellar solution. LIP activity in the reversed micelle was enhanced by optimizing either the preparation or the operation conditions, such as water content and pH in water pools of the reversed micelle and the reaction temperature. Stable activity was obtained in isooctane because of the stability of the reversed micelle. The optimal pH was 5 in the reversed micellar system, which shifted from pH 3 in the aqueous solution. The degradation reaction of several environmental pollutants was attempted using LIP hosted in the AOT reversed micelle. Degradation achieved after a 1-h reaction reached 81%, 50%, and 22% for p-nonylphenol, bisphenol A, and 2,4-dichlorophenol, respectively. This is the first report on the utilization of LIP in organic media.
ISSN:0006-3592
1097-0290
DOI:10.1002/bit.20277