Adipogenic differentiation of human induced pluripotent stem cells: Comparison with that of human embryonic stem cells

• Published in: FEBS letters 2009-03, Vol.583 (6), p.1029-1033
• Main Authors: Taura, Daisuke, Noguchi, Michio, Sone, Masakatsu, Hosoda, Kiminori, Mori, Eisaku, Okada, Yohei, Takahashi, Kazutoshi, Homma, Koichiro, Oyamada, Naofumi, Inuzuka, Megumi, Sonoyama, Takuhiro, Ebihara, Ken, Tamura, Naohisa, Itoh, Hiroshi, Suemori, Hirofumi, Nakatsuji, Norio, Okano, Hideyuki, Yamanaka, Shinya, Nakao, Kazuwa
• Format: Article
• Language: English
• Subjects: Adipocyte; Adipocytes - drug effects; Adipocytes - physiology; Adipogenesis; Adipogenesis - drug effects; Adipogenesis - genetics; Adipogenesis - physiology; Biomarkers - metabolism; CCAAT-Enhancer-Binding Proteins - genetics; CCAAT-Enhancer-Binding Proteins - metabolism; Cell Culture Techniques; Cell Differentiation - drug effects; Cell Differentiation - genetics; Cell Differentiation - physiology; Cells, Cultured; Differentiation; Embryonic Stem Cells - drug effects; Embryonic Stem Cells - metabolism; Embryonic Stem Cells - physiology; Fatty Acid-Binding Proteins - genetics; Fatty Acid-Binding Proteins - metabolism; Fibronectins - pharmacology; Gene Expression; Homeodomain Proteins - genetics; Homeodomain Proteins - metabolism; Humans; Leptin - genetics; Leptin - metabolism; Nanog Homeobox Protein; Peptides - pharmacology; Pluripotent Stem Cells - drug effects; Pluripotent Stem Cells - metabolism; Pluripotent Stem Cells - physiology; PPAR gamma - genetics; PPAR gamma - metabolism; Stem cell
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2009.02.031

Induced pluripotent stem (iPS) cells were recently established from human fibroblasts. In the present study we investigated the adipogenic differentiation properties of four human iPS cell lines and compared them with those of two human embryonic stem (ES) cell lines. After 12 days of embryoid body formation and an additional 10 days of differentiation on Poly- l-ornithine and fibronectin- coated dishes with adipogenic differentiation medium, human iPS cells exhibited lipid accumulation and transcription of adipogenesis-related molecules such as C/EBPα, PPARγ2, leptin and aP2. These results demonstrate that human iPS cells have an adipogenic potential comparable to human ES cells.