variable region of the sugarcane bacilliform virus (SCBV) genome can be used to generate promoters for transgene expression in sugarcane

Four promoters derived from sugarcane bacilliform virus (SCBV) were compared and characterised. Three were obtained by PCR amplification of purified virion DNA extracted from three sugarcane cultivars. The fourth promoter was obtained by subcloning from an almost genome-length clone of SCBV. All pro...

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Bibliographic Details
Published in:Plant cell reports 2004-11, Vol.23 (5), p.319-326
Main Authors: Braithwaite, K.S, Geijskes, R.J, Smith, G.R
Format: Article
Language:English
Subjects:
Badnavirus - genetics
Banana streak virus
beta-glucuronidase
Biological and medical sciences
Biotechnology
Cultivars
Deoxyribonucleic acid
DNA
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Gene expression
Genes, Reporter
Genetic Variation
genetic vectors
genome
Genome, Viral
kanamycin kinase
Methods. Procedures. Technologies
Miscellaneous
molecular sequence data
nucleotide sequences
Plant cells and fungal cells
Plants, Genetically Modified - genetics
promoter regions
Promoter Regions, Genetic - genetics
Proteins
quantitative analysis
Saccharum - genetics
Saccharum - virology
Saccharum officinarum
sugar crops
Sugarcane
Sugarcane bacilliform virus
transgenes
Transgenes - genetics
transgenic plants
Zea mays
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Summary:Four promoters derived from sugarcane bacilliform virus (SCBV) were compared and characterised. Three were obtained by PCR amplification of purified virion DNA extracted from three sugarcane cultivars. The fourth promoter was obtained by subcloning from an almost genome-length clone of SCBV. All promoters were able to drive stable expression of beta-glucuronidase in sugarcane. The PCR-derived promoter sequences shared more DNA homology with banana streak virus than to the subcloned SCBV. The subcloned promoter was the strongest expressing and was able to drive reporter gene expression in vitro and in the leaves, meristems and roots of glasshouse-grown sugarcane. Expression levels were at least equal to or higher than those measured for the maize polyubiquitin promoter.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-004-0817-8