The hepatic circadian clock is preserved in a lipid-induced mouse model of non-alcoholic steatohepatitis

Recent studies have correlated metabolic diseases, such as metabolic syndrome and non-alcoholic fatty liver disease, with the circadian clock. However, whether such metabolic changes per se affect the circadian clock remains controversial. To address this, we investigated the daily mRNA expression p...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2009-03, Vol.380 (3), p.684-688
Main Authors: Ando, Hitoshi, Takamura, Toshinari, Matsuzawa-Nagata, Naoto, Shima, Kosuke R., Nakamura, Seiji, Kumazaki, Masafumi, Kurita, Seiichiro, Misu, Hirofumi, Togawa, Naoyuki, Fukushima, Tatsunobu, Fujimura, Akio, Kaneko, Shuichi
Format: Article
Language:English
Subjects:
Animals
Atherogenic diet
Biological Clocks - genetics
Circadian rhythm
Circadian Rhythm - genetics
Clock gene
Diet, Atherogenic
Disease Models, Animal
Fatty Liver - genetics
Fatty Liver - physiopathology
Gene Expression Profiling
Liver - metabolism
Liver - physiopathology
Male
Mice
Mice, Inbred C57BL
Non-alcoholic steatohepatitis
Oligonucleotide Array Sequence Analysis
Oxidative stress
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Summary:Recent studies have correlated metabolic diseases, such as metabolic syndrome and non-alcoholic fatty liver disease, with the circadian clock. However, whether such metabolic changes per se affect the circadian clock remains controversial. To address this, we investigated the daily mRNA expression profiles of clock genes in the liver of a dietary mouse model of non-alcoholic steatohepatitis (NASH) using a custom-made, high-precision DNA chip. C57BL/6J mice fed an atherogenic diet for 5 weeks developed hypercholesterolemia, oxidative stress, and NASH. DNA chip analyses revealed that the atherogenic diet had a great influence on the mRNA expression of a wide range of genes linked to mitochondrial energy production, redox regulation, and carbohydrate and lipid metabolism. However, the rhythmic mRNA expression of the clock genes in the liver remained intact. Most of the circadianly expressed genes also showed 24-h rhythmicity. These findings suggest that the biological clock is protected against such a metabolic derangement as NASH.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2009.01.150