Sequence analysis of pDN571, a plasmid encoding novel bacteriocin production in M-type 57 Streptococcus pyogenes

Production of the novel bacteriocin streptococcin A-M57 (SA-M57) by Streptococcus pyogenes strains of M-protein type 57 is plasmid-associated. Plasmid pDN571 (3351 bp) harbored by S. pyogenes 71–724, the prototype M-type 57 strain, has been completely sequenced and contains three putative open readi...

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Bibliographic Details
Published in:Plasmid 2004-11, Vol.52 (3), p.225-229
Main Authors: Heng, Nicholas C.K., Burtenshaw, Grace A., Jack, Ralph W., Tagg, John R.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Bacteriocin production
Bacteriocins - genetics
M-type 57
Molecular Sequence Data
Open Reading Frames
pC194/pUB110 family
Plasmids - chemistry
Plasmids - genetics
Restriction Mapping
Rolling-circle replication
Streptococcin A-M57
Streptococcus pyogenes
Streptococcus pyogenes - genetics
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Summary:Production of the novel bacteriocin streptococcin A-M57 (SA-M57) by Streptococcus pyogenes strains of M-protein type 57 is plasmid-associated. Plasmid pDN571 (3351 bp) harbored by S. pyogenes 71–724, the prototype M-type 57 strain, has been completely sequenced and contains three putative open reading frames ( repA, scnM57 and ORF3). In addition, the double-strand and single-strand (SSO) origins of replication were identified. Analysis of the replication-associated genetic elements places pDN571 in the ubiquitous pC194/pUB110 family of rolling-circle plasmids. The SSO of pDN571 is of the ssoA type. SA-M57 (encoded by scnM57) is synthesized as a secreted 179-amino acid polypeptide with a 27-residue secretion signal peptide and has no homology to proteins of known function.
ISSN:0147-619X
1095-9890
DOI:10.1016/j.plasmid.2004.08.002