Changes in motility of the rhizobacterium Azospirillum brasilense in the presence of plant lectins

• Published in: Microbiological research 2009, Vol.164 (2), p.149-156
• Main Authors: Schelud’ko, Andrei V., Makrushin, Kirill V., Tugarova, Anna V., Krestinenko, Vyacheslav A., Panasenko, Valery I., Antonyuk, Lyudmila P., Katsy, Elena I.
• Format: Article
• Language: English
• Subjects: Azospirillum brasilense; Azospirillum brasilense - drug effects; Azospirillum brasilense - physiology; Chemotaxis - drug effects; Phaseolus vulgaris; Plant lectin; Plant Lectins - pharmacology; Solanum tuberosum; Spreading; Swarming; Swimming; Triticum aestivum; Ulex
• ISSN: 0944-5013; 1618-0623
• DOI: 10.1016/j.micres.2006.11.008

The plant-beneficial bacterium Azospirillum brasilense can swim in liquids and swarm or migrate with the formation of microcolonies in soft media. To get closer to understanding the influence of natural environments on A. brasilense motility, we studied the individual and social movement of the bacterium in the presence of various plant lectins. The lectins with specificity for N-acetyl- β- d-glucosamine oligomers (wheat germ, Solanum tuberosum and Ulex europeus agglutinins) decreased A. brasilense swimming speed and induced the formation of branched–granular colonies instead of the swarming rings. These effects seemed to be a consequence of specific interactions between the agglutinins and the lectin-binding polymers present in the A. brasilense cell envelope. Concanavalin A (with an affinity for terminal α- d-mannosyl and α- d-glucosyl residues) and Phaseolus vulgaris phytohemagglutinin P (with unknown specificity) almost did not affect the motility of A. brasilense.