Effects of dietary fish oil supplementation on cellular adhesion molecule expression and tissue myeloperoxidase activity in hypercholesterolemic mice with sepsis

• Published in: The Journal of nutritional biochemistry 2009-04, Vol.20 (4), p.254-260
• Main Authors: Chiu, Wan-Chun, Wang, Yao-Chang, Chien, Yi-Wen, Hou, Yu-Chen, Hu, Ya-Mei, Yeh, Sung-Ling
• Format: Article
• Language: English
• Subjects: Adhesion molecule; animal disease models; Animals; anti-inflammatory activity; Biological and medical sciences; cardioprotective effect; CD11b Antigen - metabolism; CD18 Antigens - metabolism; cecum; cell adhesion; Cell Adhesion Molecules - metabolism; chemoattractants; Chemokine CCL2 - metabolism; cholesteremic effect; dietary fat; Dietary Fats, Unsaturated - pharmacology; Dietary Supplements; fat intake; Feeding. Feeding behavior; Fish oil; fish oils; Fish Oils - administration & dosage; Fish Oils - pharmacology; Fundamental and applied biological sciences. Psychology; high fat diet; histopathology; Hypercholesterolemia; Hypercholesterolemia - complications; Hypercholesterolemia - enzymology; Hypercholesterolemia - immunology; immunostimulants; inflammation; Interferon-gamma - metabolism; interferons; interleukin-4; Interleukin-4 - metabolism; lymphocytes; Male; Mice; Mice, Inbred ICR; monocytes; Myeloperoxidase; neutrophils; nutrient partitioning; nutrition-genotype interaction; peroxidase; Peroxidase - metabolism; rats; Sepsis; Sepsis - etiology; Sepsis - metabolism; septicemia; tumor necrosis factor-alpha; Tumor Necrosis Factor-alpha - metabolism; Vertebrates: anatomy and physiology, studies on body, several organs or systems
• ISSN: 0955-2863; 1873-4847
• DOI: 10.1016/j.jnutbio.2008.03.001

This study investigated the effects of fish oil on adhesion molecule expression and tissue myeloperoxidase (MPO) activity in hypercholesterolemic mice with sepsis. There were one control and two experimental groups in this study. The control group (C) was fed a regular chow diet for 7 weeks, while hypercholesterolemia in the experimental group was induced by feeding a high-fat diet (20%, w/w) with cholesterol (2%, w/w) for 4 weeks. Then the experimental group was divided into two subgroups with identical nutrient distributions except that one subgroup was fed soybean oil (SO), while part of the soybean oil was replaced by fish oil (FO) in the other one for 3 weeks. After feeding the diets for 7 weeks, sepsis was induced in all three groups by cecal and ligation and puncture (CLP), and mice were sacrificed at 0, 6 or 24 h after CLP, respectively. The results showed that the FO group had a higher intracellular interferon-γ/interleukin-4 ratio and lower tumor necrosis factor-α and monocyte chemoattractant protein-1 concentrations in peritoneal lavage fluid at 6 h after CLP than those in the C and SO groups. Lymphocyte CD11a/CD18 expressions were higher at 0 and 6 h and neutrophil CD11b/CD18 were higher at 6 h in the SO group than in the FO and C groups. The SO group had higher plasma intercellular adhesion molecule (ICAM)-1 levels than C group at 0 and 6 h, whereas no difference in ICAM-1 concentrations were observed between the C and FO groups at 0 h after CLP. Hypercholesterolemia resulted in higher tissue MPO activities. There were no differences in MPO activities in various organs between the two experimental groups. These results suggest that hypercholesterolemic mice fed FO did not exhibit immunosuppression when complicated with sepsis. FO administration reduced adhesion molecule expressions and inflammatory-related mediators at the site of injury at an early but not a late stage of sepsis. However, compared with the SO group, the influences of FO on MPO activities in various organs were not obvious in hypercholesterolemic mice with sepsis.