Oligonucleotide microarray for identification of Bacillus anthracis based on intergenic transcribed spacers in ribosomal DNA

We developed a DNA microarray for identification of Bacillus anthracis and other phylogenetic groupings within the "Bacillus cereus group". Nucleotide sequences of 16S-23S ribosomal DNA internal transcribed spacers containing genes for tRNA(Ile) from 52 B. anthracis strains were found to b...

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Bibliographic Details
Published in:FEMS microbiology letters 2004-11, Vol.240 (2), p.215-223
Main Authors: Nubel, U, Schmidt, P.M, Reiss, E, Bier, F, Beyer, W, Naumann, D
Format: Article
Language:English
Subjects:
animal pathogenic bacteria
Anthrax
Bacillus anthracis
Bacillus anthracis - classification
Bacillus anthracis - genetics
Bacillus anthracis - isolation & purification
Bacillus cereus
Bacillus cereus group
Bacteriological methods and techniques used in bacteriology
Bacteriology
Base Pair Mismatch
Biological and medical sciences
Deoxyribonucleic acid
DNA
DNA chips
DNA microarrays
DNA probes
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Ribosomal Spacer - genetics
Fundamental and applied biological sciences. Psychology
Gene sequencing
Hybridization
Intergenic transcribed spacer
Microarray
microarray technology
Microbiology
Molecular Sequence Data
Nucleic Acid Hybridization
Nucleotide sequence
Nucleotides
Oligonucleotide Array Sequence Analysis
Oligonucleotides
Phylogeny
Ribosomal DNA
RNA, Bacterial - genetics
RNA, Transfer, Ile - genetics
Sensitivity and Specificity
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Spacers
Strains (organisms)
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Description
Summary:We developed a DNA microarray for identification of Bacillus anthracis and other phylogenetic groupings within the "Bacillus cereus group". Nucleotide sequences of 16S-23S ribosomal DNA internal transcribed spacers containing genes for tRNA(Ile) from 52 B. anthracis strains were found to be identical to sequences from seven strains published previously and different from all other bacteria. When 42 oligonucleotide probes targeting polymorphic sites were immobilized on glass slides and hybridized to fluorescently labeled PCR amplification products, one or more mismatches could be discriminated in all but one cases. Hence, hybridization events were highly specific and identification of B. anthracis was straightforward.
ISSN:0378-1097
1574-6968
DOI:10.1016/j.femsle.2004.09.042