Determination of quercetin in human plasma after ingestion of commercial canned green tea by semi-micro HPLC with electrochemical detection

Determination of quercetin in human plasma was carried out by semi‐micro high‐performance liquid chromatography with electrochemical detection. Peak heights for quercetin were found to be linearly related to the amount of each quercetin injected from 1.5 to 750 pg. The detection limit (signal‐noise...

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Bibliographic Details
Published in:Biomedical chromatography 2004-11, Vol.18 (9), p.662-666
Main Authors: Jin, Dongri, Hakamata, Hideki, Takahashi, Kouji, Kotani, Akira, Kusu, Fumiyo
Format: Article
Language:English
Subjects:
Adult
Chromatography, High Pressure Liquid - methods
electrochemical detection
Electrochemistry - methods
green tea
human plasma
Humans
Male
microbore column
quercetin
Quercetin - blood
Tea
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Summary:Determination of quercetin in human plasma was carried out by semi‐micro high‐performance liquid chromatography with electrochemical detection. Peak heights for quercetin were found to be linearly related to the amount of each quercetin injected from 1.5 to 750 pg. The detection limit (signal‐noise ratio, S/N = 3) of the present method for quercetin was 0.3 pg. Glucuronic and sulfate forms of quercetin in plasma were hydrolyzed enzymatically using β‐glucuronidase and sulfatase, respectively. Quercetin in plasma and the hydrolyzed solution were extracted with ethyl acetate and determined by the present method. The time courses of concentrations of quercetin in human plasma showed maxima at 1–1.5 h after ingestion of 340 mL of commercial canned green tea. Copyright © 2004 John Wiley & Sons, Ltd.
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.370