Investigation of the silylation of ephedrines using N-methyl-N-trimethylsilyl-trifluoroacetamide

This paper describes a gas chromatography (GC)--mass spectrometry (MS) method for the simultaneous quantification of ephedrine, pseudoephedrine, cathine, norephedrine and methylephedrine in urine. The sample preparation step includes solid phase extraction and derivatisation with N-methyl-N-trimethy...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2004-11, Vol.811 (2), p.201-208
Main Authors: FORSDAHL, Guro, GMEINER, Günter
Format: Article
Language:English
Subjects:
Acetamides
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Doping in Sports
Ephedrine - analysis
Ephedrine - chemistry
Fluoroacetates
Fundamental and applied biological sciences. Psychology
Gas Chromatography-Mass Spectrometry - methods
General pharmacology
Humans
Medical sciences
N-Acetylneuraminic Acid - chemistry
Pharmacology. Drug treatments
Sensitivity and Specificity
Trifluoroacetic Acid - chemistry
Trimethylsilyl Compounds - chemistry
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Summary:This paper describes a gas chromatography (GC)--mass spectrometry (MS) method for the simultaneous quantification of ephedrine, pseudoephedrine, cathine, norephedrine and methylephedrine in urine. The sample preparation step includes solid phase extraction and derivatisation with N-methyl-N-trimethylsilyl-trifluoroacetamide (MSTFA). An evaluation of various silylation conditions compatible with screening methods in doping control analysis is presented. The method was found to be well suited for quantification of ephedrines in doping control.
ISSN:1570-0232
1873-376X
DOI:10.1016/S1570-0232(04)00704-4