Association of VEGF genotype with mRNA level in colorectal adenocarcinomas

• Published in: Biochemical and biophysical research communications 2004-12, Vol.325 (1), p.144-150
• Main Authors: Yamamori, Motohiro, Sakaeda, Toshiyuki, Nakamura, Tsutomu, Okamura, Noboru, Tamura, Takao, Aoyama, Nobuo, Kamigaki, Takashi, Ohno, Masakazu, Shirakawa, Toshiro, Gotoh, Akinobu, Kuroda, Yoshikazu, Matsuo, Masafumi, Kasuga, Masato, Okumura, Katsuhiko
• Format: Article
• Language: English
• Subjects: Adenocarcinoma - genetics; Adenocarcinoma - metabolism; Base Sequence; Colorectal adenocarcinoma; Colorectal Neoplasms - genetics; Colorectal Neoplasms - metabolism; DNA Mutational Analysis; Female; Genotype; Humans; Japan; Male; Molecular Sequence Data; mRNA expression; RNA, Messenger - metabolism; Up-Regulation; Vascular Endothelial Growth Factor A - genetics; Vascular Endothelial Growth Factor A - metabolism; VEGF
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2004.10.005

The mRNA expression of vascular endothelial growth factor (VEGF) was evaluated in colorectal adenocarcinomas and adjacent noncancerous colorectal tissues in 18 Japanese patients. The expression was confirmed to be up-regulated in the colorectal adenocarcinomas, when compared with the noncancerous tissues. Twelve genotypes of VEGF: six positions in the promoter region, two in the 5′UTR, and four in the 3′UTR, and their association with the expression of VEGF mRNA were evaluated. While G-1877A, T-1455C, G-1154A, C702T, and G1612A were not detected, C-2578A, T-1498C, G-1190A, C-634G, C-7T, C936T, and C1451T were found at allele frequencies of 4/36, 15/36, 15/36, 20/36, 8/36, 6/36, and 6/36, respectively, suggesting that C-2578A, G-1154A, and G1612A were associated with a decreased risk for colorectal adenocarcinoma. T-1498C (G-1190A) and C-7T were found to be associated with higher levels of VEGF mRNA, and may be a risk factor for the development of liver metastasis and/or prognosis of colorectal adenocarcinoma.