Biocompatibility evaluation of alendronate paste in rat's subcutaneous tissue

• Published in: Dental traumatology 2009-04, Vol.25 (2), p.209-212
• Main Authors: Mori, Graziela Garrido, De Moraes, Ivaldo Gomes, Nunes, Daniele Clapes, Castilho, Lithiene Ribeiro, Poi, Wilson Roberto, Capaldi, Maria Luciana P Manzoli
• Format: Article
• Language: English
• Subjects: Alendronate - administration & dosage; Animals; Biocompatible Materials - administration & dosage; Dentistry; Disease Models, Animal; Injections, Subcutaneous; Male; Materials Testing - methods; Ointments - administration & dosage; Rats; Rats, Wistar; Root Canal Irrigants - administration & dosage; Root Resorption - drug therapy; Root Resorption - prevention & control; Subcutaneous Tissue - drug effects; Tooth Avulsion - therapy; Tooth Replantation - methods; Tooth Socket - drug effects
• ISSN: 1600-4469; 1600-9657
• DOI: 10.1111/j.1600-9657.2008.00731.x

–  Alendronate is a known inhibitor of root resorption and the development of alendronate paste would enhance its utilization as intracanal medication. Therefore, this study aimed to investigate the biocompatibility of experimental alendronate paste in subcutaneous tissue of rats, for utilization in teeth susceptible to root resorption. The study was conducted on 15 male rats, weighing ∼180–200 grams. The rats’ dorsal regions were submitted to one incision on the median region and, laterally to the incision, the subcutaneous tissue was raised and gently dissected for introduction of two tubes, in each rat. The tubes were sealed at one end with gutta‐percha and taken as control. The tubes were filled with experimental alendronate paste. The animals were killed at 7, 15 and 45 days after surgery and the specimens were processed in laboratory. The histological sections were stained with hematoxylin‐eosin and analyzed by light microscopy. Scores were assigned to the inflammatory process and statistically compared by the Tukey test (P