Rvb1p/Rvb2p Recruit Arp5p and Assemble a Functional Ino80 Chromatin Remodeling Complex

The Rvb1p and Rvb2p (or TIP48 and TIP49) nuclear ATP binding proteins are universally conserved in eukaryotes and essential for viability of yeasts. Rvbp associate with each other as a double hexamer, with YHR034c and with two complexes involved in chromatin remodeling, Ino80.com and Swr1.com. Loss...

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Bibliographic Details
Published in:Molecular cell 2004-11, Vol.16 (3), p.465-477
Main Authors: Jónsson, Zophonı́as O., Jha, Sudhakar, Wohlschlegel, James A., Dutta, Anindya
Format: Article
Language:English
Subjects:
Actin-Related Protein 2
Actins
Adenosine Triphosphatases - genetics
Adenosine Triphosphatases - metabolism
Adenosine Triphosphate - metabolism
Chromatin - genetics
Chromatin Assembly and Disassembly
DNA Helicases
RNA Helicases - genetics
RNA Helicases - metabolism
RNA, Fungal - analysis
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Saccharomyces cerevisiae Proteins - metabolism
Transcription Factors
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Summary:The Rvb1p and Rvb2p (or TIP48 and TIP49) nuclear ATP binding proteins are universally conserved in eukaryotes and essential for viability of yeasts. Rvbp associate with each other as a double hexamer, with YHR034c and with two complexes involved in chromatin remodeling, Ino80.com and Swr1.com. Loss of Rvb1p or Ino80p affects many yeast promoters similarly. Rvbp are not essential for the recruitment of Ino80p to promoters but are essential for the catalytic activity of Ino80.com. Loss of Rvbp leads to loss of the functionally critical Arp5p in Ino80.com. Rvb2p associates with Arp5p in vitro in a reaction dependent on the presence of ATP and Ino80p. Therefore, Rvbp are required for the structural and functional integrity of the Ino80 chromatin remodeling complex.
ISSN:1097-2765
1097-4164
DOI:10.1016/j.molcel.2004.09.033