A cellulose synthase-containing compartment moves rapidly beneath sites of secondary wall synthesis

The woody secondary walls of plants represent the major sites of cellulose deposition. The polymerization of cellulose occurs at the plasma membrane by the secondary wall cellulose synthase complex (CSC). In the long, cylindrical cells that make up the xylem, secondary wall deposition is confined to...

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Bibliographic Details
Published in:Plant and cell physiology 2009-03, Vol.50 (3), p.584-594
Main Authors: Wightman, R.(University of Manchester (UK)), Marshall, R, Turner, S.R
Format: Article
Language:English
Subjects:
ARABIDOPSIS
Arabidopsis - enzymology
Arabidopsis Proteins - metabolism
BIOSINTESIS
BIOSYNTHESE
BIOSYNTHESIS
Cell Wall - metabolism
CELLULOSE
Cellulose - metabolism
Cellulose synthase complex
CELULOSA
FLIP
Fluorescence
Glucosyltransferases - metabolism
Photobleaching
POLIMERIZACION
POLYMERISATION
POLYMERIZATION
Secondary cell wall
XILEMA
XYLEM
Xylem - cytology
Xylem - metabolism
XYLEME
YFP
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Summary:The woody secondary walls of plants represent the major sites of cellulose deposition. The polymerization of cellulose occurs at the plasma membrane by the secondary wall cellulose synthase complex (CSC). In the long, cylindrical cells that make up the xylem, secondary wall deposition is confined to discrete regions of the cell, and yellow fluorescent protein (YFP)-labeled CSCs are also localized to these regions. Using fluorescence loss in photobleaching (FLIP) of complete hoops containing YFP-CSCs, we demonstrate movement of the complexes beneath the nascent secondary wall in developing xylem vessels. We have devised a method for determining particle velocities for particles moving around a cylindrical object using data from FLIP. By applying this method to the hoops of YFP-CSCs of the developing vessels, we have obtained the first estimates of speed of these complexes. These speeds are calculated to be in excess of 7 microm/s and are far higher than those speeds previously reported for the primary wall complex. These high speeds are unlikely to be consistent with CSC movement being attributed to cellulose synthesis alone, and suggest the existence of a highly motile compartment beneath the nascent secondary wall.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pcp017