Comparative analysis of T-cell costimulation and CD43 activation reveals novel signaling pathways and target genes

The CD43 lymphocyte surface receptor is involved in the regulation of lymphocyte adhesion and activation. Many CD43 functions remain controversial or unclear, and it is not known to which extent CD43 signaling pathways are shared with or distinct from those used by the T-cell receptor (TCR). Here, w...

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Bibliographic Details
Published in:Blood 2004-11, Vol.104 (10), p.3302-3304
Main Authors: Mattioli, Ivan, Dittrich-Breiholz, Oliver, Livingstone, Mark, Kracht, Michael, Schmitz, M. Lienhard
Format: Article
Language:English
Subjects:
Antigens, CD - metabolism
Biological and medical sciences
CD28 Antigens - metabolism
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Gene Expression Regulation - immunology
Humans
Immunobiology
JNK Mitogen-Activated Protein Kinases - metabolism
Leukosialin
Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation
NF-kappa B - metabolism
Oligonucleotide Array Sequence Analysis
Sialoglycoproteins - metabolism
Signal Transduction - immunology
T-Lymphocytes - immunology
T-Lymphocytes - metabolism
Transcription Factor RelA
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Summary:The CD43 lymphocyte surface receptor is involved in the regulation of lymphocyte adhesion and activation. Many CD43 functions remain controversial or unclear, and it is not known to which extent CD43 signaling pathways are shared with or distinct from those used by the T-cell receptor (TCR). Here, we systematically compared signaling events and target gene expression induced by CD43 or T-cell costimulation in primary human peripheral T cells. These studies identify nuclear factor-κB (NF-κB) p65 serine 468 as a novel inducible phosphorylation site strongly induced by T-cell costimulation and only weakly triggered by CD43 ligation. We also identified CD43 as a novel Jun N-terminal kinase (JNK) activator and a comprehensive analysis of further signaling events suggests that both stimuli use overlapping but also distinct signaling pathways. Microarray analysis of inflammatory genes shows 1 group of genes coregulated by both stimuli and 2 further groups of target genes affected solely by costimulation or primarily by CD43. (Blood. 2004;104:3302-3304)
ISSN:0006-4971
1528-0020
DOI:10.1182/blood-2004-04-1536