An LEA group 3 family member is involved in survival of C. elegans during exposure to stress

In order to establish a functional role for late embryogenesis abundant (LEA) proteins in response to stress conditions in Caenorhabditis elegans, we silenced the expression of an LEA ( Ce-lea-1) gene and determined the survival of worms under stress conditions. Ce-lea-1 transcription was induced du...

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Bibliographic Details
Published in:FEBS letters 2004-11, Vol.577 (1), p.21-26
Main Authors: Gal, Tali Z., Glazer, Itamar, Koltai, Hinanit
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Caenorhabditis elegans - physiology
Caenorhabditis elegans Proteins - genetics
Caenorhabditis elegans Proteins - physiology
Ce-lea-1
Ce-lin-53
Ce-unc-120
Dehydration - physiopathology
Desiccation stress
DJ, dauer juveniles
DNA Primers
Gene Silencing
Heat stress
Heat Stress Disorders
L1, first-stage larva
L4, fourth-stage larva
LEA, late embryogenesis abundant
Osmotic Pressure
Osmotic stress
Reverse Transcriptase Polymerase Chain Reaction
RH, relative humidity
RNAi, RNA interference
Transcription, Genetic
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Summary:In order to establish a functional role for late embryogenesis abundant (LEA) proteins in response to stress conditions in Caenorhabditis elegans, we silenced the expression of an LEA ( Ce-lea-1) gene and determined the survival of worms under stress conditions. Ce-lea-1 transcription was induced during dehydration of C. elegans dauer juveniles. Following partial silencing of Ce-lea-1 transcription, we demonstrated a specific and significant reduction in worm survival during induction of desiccation, osmotic and heat stress. Together, these results establish a functional role for Ce-lea-1 in stress survival of C. elegans and suggest that Ce-lea-1 may function as a component that is common to the responses to the examined stress conditions.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2004.09.049