Conserved transcriptional regulation of a cone phototransduction gene in vertebrates

cGMP-phosphodiesterase (PDE) is a key component in visual phototransduction. Rod and cone photoreceptors each produce their unique cGMP-PDE subunits. The α ′ catalytic subunits are believed to be cone-specific. In this study, we report that transfection of the −132 to +139 sequence in the upstream r...

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Bibliographic Details
Published in:FEBS letters 2004-11, Vol.577 (1), p.259-264
Main Authors: Viczian, A.S., Verardo, M., Zuber, M.E., Knox, B.E., Farber, D.B.
Format: Article
Language:English
Subjects:
Animals
Animals, Genetically Modified
Anura
Base Sequence
Cone
DNA Primers
Electrophoretic Mobility Shift Assay
Gene Expression Regulation
GFP, green fluorescent protein
Immunohistochemistry
Light
PDE, cyclic GMP-phosphodiesterase
Phosphodiesterase
Photoreceptor
Retina
Signal Transduction - genetics
Transcription
Transcription, Genetic
Vertebrates - genetics
Xenopus
Xenopus laevis
α′-PDE, alpha prime catalytic subunit of cyclic GMP-phosphodiesterase
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Summary:cGMP-phosphodiesterase (PDE) is a key component in visual phototransduction. Rod and cone photoreceptors each produce their unique cGMP-PDE subunits. The α ′ catalytic subunits are believed to be cone-specific. In this study, we report that transfection of the −132 to +139 sequence in the upstream region of the human α ′-PDE gene fused to luciferase cDNA gives the highest level of reporter gene transcription in cultured retinoblastoma Y79 cells. Transgenic Xenopus laevis carrying this sequence fused to green fluorescent protein (GFP) expressed GFP in cones, suggesting a conserved regulatory mechanism for α ′-PDE transcription in both human and frog.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2004.10.008