Immunohistochemical Localization of Glycogen Phosphorylase Isozymes in the Rat Gastrointestinal Muscle Layers and Enteric Nervous System

Glycogen represents the major brain energy reserve though its precise functions are still under debate. Glycogen has also been found in different cell types of the enteric nervous system (ENS), the largest and most complex component of the peripheral nervous system. In the present work we have demon...

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Bibliographic Details
Published in:Neurochemical research 2009-05, Vol.34 (5), p.876-883
Main Authors: Pfeiffer-Guglielmi, Brigitte, Francke, Mike, Roski, Christian, Hanani, Menachem, Reichenbach, Andreas, Hamprecht, Bernd
Format: Article
Language:English
Subjects:
Animals
Biochemistry
Biomedical and Life Sciences
Biomedicine
Blotting, Western
Cell Biology
Enteric Nervous System - enzymology
Female
Gastrointestinal Tract - enzymology
Gastrointestinal Tract - innervation
Glycogen Phosphorylase, Brain Form - metabolism
Glycogen Phosphorylase, Muscle Form - metabolism
Immunohistochemistry
Intestine, Large - enzymology
Intestine, Large - innervation
Intestine, Small - enzymology
Intestine, Small - innervation
Isoenzymes - metabolism
Male
Muscle, Smooth - enzymology
Neurochemistry
Neurology
Neurosciences
Organ Specificity
Original Paper
Rats
Rats, Wistar
Stomach - enzymology
Stomach - innervation
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Summary:Glycogen represents the major brain energy reserve though its precise functions are still under debate. Glycogen has also been found in different cell types of the enteric nervous system (ENS), the largest and most complex component of the peripheral nervous system. In the present work we have demonstrated, by application of isozyme-specific antibodies, the presence of isozymes of glycogen phosphorylase (GP), one of the major control sites in glycogen metabolism, in the rat ENS. Immunohistochemistry revealed that isoform BB (brain) is the predominant isozyme expressed in enteric glial cells (EGC) and rare neurons of the myenteric and submucosal plexuses. Isoform MM (muscle) appears in cells which are, according to their location and morphology, probably interstitial cells of Cajal (ICC). In addition, both GP isoforms are expressed in longitudinal and circular intestinal smooth muscle layers. As GP BB is mainly regulated by the cellular AMP level, a special function of glycogen in the energy supply of neural gut functions is suggested.
ISSN:0364-3190
1573-6903
DOI:10.1007/s11064-008-9834-2