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Transcriptional response to glucose starvation and functional analysis of a glucose transporter of Neurospora crassa
The response to glucose availability in Neurospora crassa was characterized by measuring transcript levels of 1335 genes represented on a cDNA microarray. We found that 19% of the 1335 genes were regulated at least twofold in response to glucose deprivation. Several sugar transporter homologs were f...
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| Published in: | Fungal genetics and biology 2004-12, Vol.41 (12), p.1104-1119 |
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| Main Authors: | , , , , , |
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| container_end_page | 1119 |
| container_issue | 12 |
| container_start_page | 1104 |
| container_title | Fungal genetics and biology |
| container_volume | 41 |
| creator | Xie, Xin Wilkinson, Heather H. Correa, Alejandro Lewis, Zachary A. Bell-Pedersen, Deborah Ebbole, Daniel J. |
| description | The response to glucose availability in
Neurospora crassa was characterized by measuring transcript levels of 1335 genes represented on a cDNA microarray. We found that 19% of the 1335 genes were regulated at least twofold in response to glucose deprivation. Several sugar transporter homologs were found to be regulated by glucose. Functional analysis of one of these,
hgt-1, indicates that it encodes a high-affinity glucose transporter. Comparing
N. crassa profiling data with the published diauxic shift data from
Saccharomyces cerevisiae revealed that the two fungi share similar, but not identical, transcriptional response patterns. Regulation of transcript levels measured by microarray analysis is consistent with previous measurements of central metabolism enzyme activities for
N. crassa. Transcripts of genes for ethanol fermentation and the tricarboxylic acid cycle are abundant in glucose-rich medium, consistent with
N. crassa carrying out a high rate of both fermentation and respiration simultaneously. |
| doi_str_mv | 10.1016/j.fgb.2004.08.009 |
| format | article |
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hgt-1, indicates that it encodes a high-affinity glucose transporter. Comparing
N. crassa profiling data with the published diauxic shift data from
Saccharomyces cerevisiae revealed that the two fungi share similar, but not identical, transcriptional response patterns. Regulation of transcript levels measured by microarray analysis is consistent with previous measurements of central metabolism enzyme activities for
N. crassa. Transcripts of genes for ethanol fermentation and the tricarboxylic acid cycle are abundant in glucose-rich medium, consistent with
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Neurospora crassa was characterized by measuring transcript levels of 1335 genes represented on a cDNA microarray. We found that 19% of the 1335 genes were regulated at least twofold in response to glucose deprivation. Several sugar transporter homologs were found to be regulated by glucose. Functional analysis of one of these,
hgt-1, indicates that it encodes a high-affinity glucose transporter. Comparing
N. crassa profiling data with the published diauxic shift data from
Saccharomyces cerevisiae revealed that the two fungi share similar, but not identical, transcriptional response patterns. Regulation of transcript levels measured by microarray analysis is consistent with previous measurements of central metabolism enzyme activities for
N. crassa. Transcripts of genes for ethanol fermentation and the tricarboxylic acid cycle are abundant in glucose-rich medium, consistent with
N. crassa carrying out a high rate of both fermentation and respiration simultaneously.</description><subject>Adaptation, Physiological - genetics</subject><subject>Amino Acid Sequence</subject><subject>Citric Acid Cycle - genetics</subject><subject>Conserved Sequence</subject><subject>DNA, Complementary</subject><subject>DNA, Fungal - genetics</subject><subject>Energy Metabolism - genetics</subject><subject>Ethanol</subject><subject>Fungal Proteins - physiology</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Gluconeogenesis</subject><subject>Glucose - metabolism</subject><subject>Glycolysis</subject><subject>Microarray</subject><subject>Molecular Sequence Data</subject><subject>Monosaccharide Transport Proteins - genetics</subject><subject>Monosaccharide Transport Proteins - physiology</subject><subject>Neurospora crassa</subject><subject>Neurospora crassa - genetics</subject><subject>Neurospora crassa - metabolism</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>RNA, Fungal - analysis</subject><subject>RNA, Messenger - analysis</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sugar transport</subject><subject>TCA cycle</subject><subject>Transcription, Genetic</subject><issn>1087-1845</issn><issn>1096-0937</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqFkU9v1DAQxSMEov_4AFwgJ24J49iOY3FCFS1IFRzani3HHq-8ysbBdir12-NoV-UGF3tG_r2nGb-qek-gJUD6z_vW7ca2A2AtDC2AfFWdE5B9A5KK11s9iIYMjJ9VFyntAQjhjLytzgjnlHSEnVf5Ieo5meiX7MOspzpiWsKcsM6h3k2rCaVMWccnvQG1nm3t1tmcaF2O5-RTHVytX_i8eS4hZozbw09cY9h6XZuoU9JX1Runp4TvTvdl9Xjz7eH6e3P36_bH9de7xjBgueHCCsLRjQh2kBQY58ZZwx1y4UwnOirdWLYGRE0tOtP3HKVjemSOW6vpZfXp6LvE8HvFlNXBJ4PTpGcMa1K9ACY7Sv8LEiEYhY4XkBxBUzZKEZ1aoj_o-KwIqC0TtVclE7VlomBQZbqi-XAyX8cD2r-KUwgF-HgEnA5K76JP6vG-A0KLWkpCN4svRwLLbz15jCoZj7NB6yOarGzw_xjgD82MqWs</recordid><startdate>20041201</startdate><enddate>20041201</enddate><creator>Xie, Xin</creator><creator>Wilkinson, Heather H.</creator><creator>Correa, Alejandro</creator><creator>Lewis, Zachary A.</creator><creator>Bell-Pedersen, Deborah</creator><creator>Ebbole, Daniel J.</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20041201</creationdate><title>Transcriptional response to glucose starvation and functional analysis of a glucose transporter of Neurospora crassa</title><author>Xie, Xin ; 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Neurospora crassa was characterized by measuring transcript levels of 1335 genes represented on a cDNA microarray. We found that 19% of the 1335 genes were regulated at least twofold in response to glucose deprivation. Several sugar transporter homologs were found to be regulated by glucose. Functional analysis of one of these,
hgt-1, indicates that it encodes a high-affinity glucose transporter. Comparing
N. crassa profiling data with the published diauxic shift data from
Saccharomyces cerevisiae revealed that the two fungi share similar, but not identical, transcriptional response patterns. Regulation of transcript levels measured by microarray analysis is consistent with previous measurements of central metabolism enzyme activities for
N. crassa. Transcripts of genes for ethanol fermentation and the tricarboxylic acid cycle are abundant in glucose-rich medium, consistent with
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| ispartof | Fungal genetics and biology, 2004-12, Vol.41 (12), p.1104-1119 |
| issn | 1087-1845 1096-0937 |
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| source | ScienceDirect Journals |
| subjects | Adaptation, Physiological - genetics Amino Acid Sequence Citric Acid Cycle - genetics Conserved Sequence DNA, Complementary DNA, Fungal - genetics Energy Metabolism - genetics Ethanol Fungal Proteins - physiology Gene Expression Profiling Gene Expression Regulation, Fungal Gluconeogenesis Glucose - metabolism Glycolysis Microarray Molecular Sequence Data Monosaccharide Transport Proteins - genetics Monosaccharide Transport Proteins - physiology Neurospora crassa Neurospora crassa - genetics Neurospora crassa - metabolism Oligonucleotide Array Sequence Analysis RNA, Fungal - analysis RNA, Messenger - analysis Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Sequence Alignment Sequence Homology, Amino Acid Sugar transport TCA cycle Transcription, Genetic |
| title | Transcriptional response to glucose starvation and functional analysis of a glucose transporter of Neurospora crassa |
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