Evaluation of hyaluronan from different sources: Streptococcus zooepidemicus, rooster comb, bovine vitreous, and human umbilical cord

Sodium hyaluronate (HA) is widely distributed in extracellular matrixes and can play a role in orchestrating cell function. Consequently, many investigators have looked at the effect of exogenous HA on cell behavior in vitro. HA can be isolated from several sources (e.g., bacterial, rooster comb, um...

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Bibliographic Details
Published in:Biomacromolecules 2004-11, Vol.5 (6), p.2122-2127
Main Authors: SHIEDLIN, Aviva, BIGELOW, Russell, CHRISTOPHER, William, ARBABI, Saman, YANG, Laura, MATER, Ronald V, WAINWRIGHT, Norman, CHILDS, Alice, MILLER, Robert J
Format: Article
Language:English
Subjects:
Animals
Applied sciences
Cattle
Chickens
Chromatography, High Pressure Liquid
Comb and Wattles
Electrophoresis, Polyacrylamide Gel
Endotoxins - metabolism
Exact sciences and technology
Humans
Hyaluronic Acid - chemistry
Macrophage Activation
Macrophages - metabolism
Natural polymers
Nucleic Acids - metabolism
Physicochemistry of polymers
Polymers - chemistry
Powders
Rabbits
RNA - chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Starch and polysaccharides
Streptococcus
Streptococcus equi - metabolism
Swine
Time Factors
Tumor Necrosis Factor-alpha - metabolism
Umbilical Cord - metabolism
Umbilical Veins - cytology
Vitreous Body - metabolism
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Summary:Sodium hyaluronate (HA) is widely distributed in extracellular matrixes and can play a role in orchestrating cell function. Consequently, many investigators have looked at the effect of exogenous HA on cell behavior in vitro. HA can be isolated from several sources (e.g., bacterial, rooster comb, umbilical cord) and therefore can possess diverse impurities. This current study compares the measured impurities and the differences in biological activity between HA preparations from these sources. It was demonstrated that nucleic acid and protein content was highest in human umbilical cord and bovine vitreous HA and was low in bacterial and rooster comb HA. Macrophages exposed to human umbilical cord HA produced significantly higher amounts of TNF-alpha relative to control or bacterial-derived HA. These results indicate that the source of HA should be considered due to differences in the amounts and types of contaminants that could lead to widely different behaviors in vitro and in vivo.
ISSN:1525-7797
1526-4602
DOI:10.1021/bm0498427